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. 2012 Jan;132(1):246-9.
doi: 10.1038/jid.2011.267. Epub 2011 Aug 18.

Transcriptional profiling of psoriasis using RNA-seq reveals previously unidentified differentially expressed genes

Ali Jabbari, Mayte Suárez-Fariñas, Scott Dewell, James G Krueger

Transcriptional profiling of psoriasis using RNA-seq reveals previously unidentified differentially expressed genes

Ali Jabbari et al. J Invest Dermatol. 2012 Jan.
No abstract available

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Conflict of interest statement

Conflict of Interest

James G. Krueger has received honoraria and served as a consultant for Amgen, Centocor, Janssen, Ortho Biotech, Merck, Pfizer, Biogen Idec, and GlaxoSmithKline.

Figures

Figure 1
Figure 1. Identification of a large set of differentially expressed genes in psoriatic skin
(a) Venn diagram comparing DEGs between lesional and non-lesional skin of psoriatic patients as assessed by RNA-seq and microarray analysis using the samples from the same set of patients. Indicated in the diagram are the numbers of upregulated and downregulated DEGs. (b) Boxplots (means, boxes 25–75%, +/− whiskers 95%) of the absolute values of the log2 fold change among ten quantiles of concentrations for RNA-seq (left panel) and microarray analyses (right panel). (c) Scatterplot of the FC values for all genes as estimated by RNA-seq and microarray analyses. (d) Genes were grouped based on dot plot location (left panel) and assessed for mean expression as determined by microarray analysis (right panel).
Figure 2
Figure 2. RNA-seq identifies a novel set of DEGs in psoriasis skin lesions not otherwise identified in prior published studies
(a) Venn diagram comparing DEGs identified by RNA-seq and those identified by published microarray studies. (b) Real time RT-PCR confirmation of six upregulated (upper panel) and six downregulated (lower panel) DEGs identified by RNA-seq. Displayed are mean log2 fold change +/− SEM, 15 paired lesional and control nonlesional samples for each gene. (c) SPRR2B protein expression was detected in psoriatic nonlesional (left) and lesional (right) skin sections by immunohistochemistry (100x magnification, three sets of paired samples assessed, scale bar is equal to 200 micrometers).
See this image and copyright information in PMC

References

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