Molecular epidemiology of clinical isolates of Pseudomonas aeruginosa isolated from horses in Ireland

Abstract

Clinical isolates (n = 63) of Pseudomonas aeruginosa obtained from various sites in 63 horses were compared using ERIC2 RAPD PCR to determine their genetic relatedness. Resulting banding patterns (n = 24 genotypes) showed a high degree of genetic heterogeneity amongst all isolates examined, indicating a relative non-clonal relationship between isolates from these patients, employing this genotyping technique. This study characterised 63 clinical isolates into 24 distinct genotypes, with the largest cluster (genotype E) accounting for 10/63 (15.9%) of the isolates. ERIC2 RAPD PCR proved to be a highly discriminatory molecular typing tool of P. aeruginosa in isolates recovered from horses. With the adoption of several controls to aid reproducibility, this technique may be useful as an alternative to PFGE, particularly in epidemiological investigations of outbreaks where speed may be a significant parameter. This is the first report of clonal heterogeneity amongst P. aeruginosa from horses and demonstrated that ERIC RAPD PCR is a rapid method for the examination of this species in horses, which may be useful in outbreak analysis.

Figure 1

Representative banding profiles associated with ERIC2 RAPD PCR in P. aeruginosa isolated from horses. Lane M: Molecular weight marker (100 bp); lane 1: genotype A; lanes 2 and 3, genotype B; lanes 4, 5 and 9, genotype C; lane 6, genotype D; lanes 7 and 13, genotype E; lane 8, genotype F; lane 10, genotype G; lane 11, genotype H; lane 12, genotype H; lane 14, genotype J; lane 15, genotype K; lane 16, genotype L; lane 17, genotype M; lane 18, genotype N.