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. 2011 Aug 19:11:222.
doi: 10.1186/1471-2334-11-222.

Prevalence and antimicrobial resistance of Salmonella isolated from lactating cows and in contact humans in dairy farms of Addis Ababa: a cross sectional study

Zelalem Addis  1 , Nigatu Kebede, Zufan Worku, Haile Gezahegn, Alehegne Yirsaw, Tesfu Kassa
Affiliations

Prevalence and antimicrobial resistance of Salmonella isolated from lactating cows and in contact humans in dairy farms of Addis Ababa: a cross sectional study

Zelalem Addis et al. BMC Infect Dis. .

Abstract

Background: Salmonella are the major pathogenic bacteria in humans as well as in animals. Salmonella species are leading causes of acute gastroenteritis in several countries and salmonellosis remains an important public health problem worldwide, particularly in the developing countries. The situation is more aggravated by the ever increasing rate of antimicrobial resistance strains. Cattle have been implicated as a source of human infection with antimicrobial resistant Salmonella through direct contact with livestock and through the isolation of antimicrobial resistant Salmonella from raw milk, cheddar cheese, and hamburger meat traced to dairy farms. Despiite the presence of many studies on the prevalence and antimicrobial susceptibility pattern of Salmonella in Ethiopia, nothing has been said on the degree of the situation among apparently healthy lactating cows and in contact humans. Hence this study was conducted to determine the prevalence and antimicrobial resistance pattern of Salmonella isolates from lactating cows and in contact humans in dairy farms of Addis Ababa.

Methods: a cross sectional study was conducted in Addis Ababa by collecting milk and faecal samples from lactating cows and stool samples from humans working in dairy farms. Samples were pre-enriched in buffered peptone water followed by selective enrichment using selenite cysteine and Rapaport-Vassilidis broths. Isolation and identification was made by inoculating the selectively enriched sample on to Xylose Lysine Deoxycholate agar followed by confirmation of presumptive colonies using different biochemical tests. The Kibry Bauer disk diffusion method was used for antimicrobial sensitivity testing.

Results: 10.7% (21/195) of cows and 13.6% (3/22) of the human subjects sheded Salmonella. 83% resistance to two or more antimicrobials and 100% resistance to ampicillin were observed. Most of the isolates were relatively sensitive to ciprofloxacin, cotrimoxazole, and chloramphenicol.

Conclusion: High proportion of Salmonella isolates developed resistance to the commonly prescribed antimicrobials and this may be a considerable risk in the treatment of clinical cases. So, wise use of antimicrobials must be practiced to combat the ever increasing situation of antimicrobial resistance.

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Figures

Figure 1
Figure 1
Percentage activity of antimicrobials tested against Salmonella isolates from lactating dairy cows and individuals working in the dairy farms.
See this image and copyright information in PMC

References

    1. Rotimi VO, Jamal W, Pal T, Sonnevend A, Dimitrov TS, Albert MJ. Emergence of multidrug-resistant Salmonella spp. and isolates with reduced susceptibility to ciprofloxacin in Kuwait and the United Arab Emirates. Diagn Microbiol Infect Dis. 2008;60:71–7. - PubMed
    1. Stevens A, Kabore Y, Perrier-Gros-Claude JD, Millemann Y, Brisabois A, Catteau M, Cavin J, Dufour B. Prevalence and antibiotic-resistance of Salmonella isolated from beef sampled from the slaughterhouse and from retailers in Dakar (Senegal) Int J Food Microbiol. 2006;110:178–86. doi: 10.1016/j.ijfoodmicro.2006.04.018. - DOI - PubMed
    1. Ponce E, Khan AA, Cheng CM, Summage WC, Cerniglia CE. Prevalence and characterization of Salmonella enteric serovar Weltevreden from imported seafood. Food Microbiology. 2008;25:29–35. doi: 10.1016/j.fm.2007.09.001. - DOI - PubMed
    1. Molla B, Mesfin A, Alemayehu D. Multiple antimicrobial resistant Salmonella serotypes isolated from chicken carcases and giblets in Debre Zeit and Addis Ababa, Ethiopia. Ethiop J Health Dev. 2003;17:131–149.
    1. Molla W, Molla B, Alemayehu D, Muckle A, Cole L, Wilkie E. Occurrence and antimicrobial resistance of Salmonella serovars in apparently healthy slaughtered sheep and goats of central Ethiopia. Trop Anim Health Prod. 2006;38:455–62. doi: 10.1007/s11250-006-4325-4. - DOI - PubMed

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