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. 2012 Feb 15;37(4):E209-15.
doi: 10.1097/BRS.0b013e31822ce81f.

Alterations in T2 relaxation magnetic resonance imaging of the ovine intervertebral disc due to nonenzymatic glycation

Ehsan Jazini  1 , Alok D Sharan, Lee Jae Morse, Jonathon P Dyke, Eric B Aronowitz, Louis K H Chen, Simon Y Tang
Affiliations

Alterations in T2 relaxation magnetic resonance imaging of the ovine intervertebral disc due to nonenzymatic glycation

Ehsan Jazini et al. Spine (Phila Pa 1976). .

Abstract

Study design: An in vitro study using ovine intervertebral discs to correlate the effects of increasing advanced glycation end-products (AGEs) with disc hydration evaluated by magnetic resonance imaging (MRI).

Objective: To determine the relationship between the level of AGEs and tissue water content in intervertebral discs using T2 relaxation MRI.

Summary of background data: AGEs result from nonenzymatic glycation, and AGEs have been shown to accumulate in the intervertebral disc tissue with aging and degeneration. AGEs can alter biochemical properties, including the hydrophobicity of the extracellular matrix. Because one of the degenerative signs of the intervertebral disc (IVD) is reduced hydration, we hypothesized that increased levels of tissue AGEs contribute to disc hydration. T2 relaxation MRI has been shown to be sensitive to the hydration status of the disc and may be valuable in detecting the changes in the IVD mediated by the increase of AGEs.

Methods: Thirty-eight IVDs were obtained from 4 ovine spines, and the annulus fibrosis (AF) and nucleus pulposus (NP) tissues were isolated from these discs. The tissues were incubated in either a ribosylation or control solution for up to 8 days to induce the formation of AGEs. T2 relaxation times were obtained from these tissues after ribosylation. These tissues were subsequently analyzed for hydration, proteoglycan, collagen, and AGEs content.

Results: In vitro ribosylation led to the increased accumulation of AGEs and reduced water content in both the AF and NP in a dose-dependent manner, but did not affect the proteoglycan and collagen composition. When analyzed by MRI, ribosylation significantly altered the mean T2 relaxation times in the NP (P = 0.001), but not in the AF (P = 0.912). Furthermore, the mean T2 values in the NP significantly decreased with increasing periods of incubation time (P < 0.001).

Conclusion: This study demonstrates that levels of AGEs in the IVD may affect the tissue water content. Moreover, these ribosylation-mediated changes in tissue hydration were detectable using T2 relaxation MRI. T2 relaxation MRI may provide a noninvasive tool to measure in vivo changes in disc hydration that are negatively correlated with the accumulation of AGEs.

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Figures

Figure 1
Figure 1
(A) The intervertebral disc tissues were dissected from the annulus fibrosus (AF) and nucleus pulposus (NP). (B) A schematic illustrating the allocation of sample sizes in each experiment.
Figure 2
Figure 2
(A) The accumulation of AGEs increased with ribosylation incubation time in both the annulus fibrosis (p<0.001; ANOVA) and the nucleus pulposus (p<0.001; ANOVA). (B) The increases in AGEs also corresponded with the decrease in the tissue water mass in these IVD tissues. Water content in the intervertebral disc tissues decreased significantly with incubation time in both the annulus fibrosis (p<0.001; ANOVA) and the nucleus pulposus (p<0.001; ANOVA).
Figure 3
Figure 3
The loss of water content in the intervertebral disc tissues was significantly correlated with the accumulation of AGEs in both the annulus and the nucleus.
Figure 4
Figure 4
Representative T2 relaxation distributions of AF and NP samples at 0 days of ribosylation (control) and at 8 days of ribosylation. Ribosylation has little effect on the MR T2 relaxation times of the annulus fibrosus samples, while reducing the MR T2 relaxation times of the nucleus pulposus samples.
Figure 5
Figure 5
NP tissues exhibited a loss in T2 relaxation times in a dose-dependent manner with increasing ribosylation times (p<0.001; Pearson’s correlation).
See this image and copyright information in PMC

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