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. 2011 Nov;55(11):5403-7.
doi: 10.1128/AAC.00585-11. Epub 2011 Aug 22.

Genetic features of blaNDM-1-positive Enterobacteriaceae

Laurent Poirel  1 , Laurent Dortet, Sandrine Bernabeu, Patrice Nordmann
Affiliations

Genetic features of blaNDM-1-positive Enterobacteriaceae

Laurent Poirel et al. Antimicrob Agents Chemother. 2011 Nov.

Abstract

Genetic features associated with the bla(NDM-1) gene were investigated in 6 Escherichia coli, 7 Klebsiella pneumoniae, 1 Citrobacter freundii, 1 Proteus mirabilis, and 1 Providencia stuartii isolate of worldwide origin. Clonal diversity was observed for both E. coli and K. pneumoniae. The bla(NDM-1) gene was carried by different plasmid types (IncA/C, IncF, IncL/M, or untypeable) and was likely chromosome borne in two isolates. The bla(NDM-1) plasmids coharbored a variety of resistance determinants, including β-lactamase genes, quinolone resistance genes, and 16S RNA methylase genes.

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Figures

Fig. 1.
Fig. 1.
Worldwide distribution of NDM-1-producing E. coli and K. pneumoniae isolates. The color codes for the isolates are used to indicate the country where the isolate was recovered. Circles, E. coli; stars, K. pneumoniae. Isolates include those identified in the present study (underlined STs) and strains reported in other studies only when the results of MLST typing were available (, , –20, 22, 25, 29).
Fig. 2.
Fig. 2.
Schematic map of blaNDM-1-associated genetic structures identified among enterobacterial isolates. (A) Structure identified from E. coli 271 in which the ISEc33 and ISSen4 elements have been identified on both ends of the blaNDM-1 gene (19); (B)structure in which ISAba125 is present as a truncated element, but without ISEc33 (K. pneumoniae Kp7 and 601); (C) structure in which ISAba125 is present as a full element and the bleMBL gene is present (E. coli GUE, I5, RKI, and 5649; K. pneumoniae K2, IBN, and 419; Citrobacter freundii STE, and P. stuartii PS1); (D) structure initially identified in K. pneumoniae 05-506 (29); (E) structure in which ISAba125 is present as a full element but the bleomycin resistance bleMBL gene is absent or truncated (E. coli RIC, K. pneumoniae 6642 and 6759, Proteus mirabilis 7892). Genes and their corresponding transcription orientations are indicated by horizontal arrows. Positions of the primers as well as the blaNDM-1 promoter are indicated.
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References

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