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Review
. 2011 Jul-Aug;77(4):531-536.
doi: 10.1590/S1808-86942011000400020.

TNF-R2 expression in acquired middle ear cholesteatoma

[Article in English, Portuguese]
Rodrigo Faller Vitale  1 , Celina Siqueira Barbosa Pereira  2 , Adriana Leal Alves  3 , Jose Humberto Tavares Guerreiro Fregnani  4 , Fernando Quintanilha Ribeiro  5
Affiliations
Review

TNF-R2 expression in acquired middle ear cholesteatoma

[Article in English, Portuguese]
Rodrigo Faller Vitale et al. Braz J Otorhinolaryngol. 2011 Jul-Aug.

Abstract

Acquired middle ear cholesteatoma is a disease which promotes bone erosion resulting in potentially serious complications. The tumor necrosis factor alpha (TNF-α) is present in cholesteatoma and it is related to bone erosion, as shown by different authors. To understand the aggressiveness characteristics of cholesteatoma is necessary, however, to better address the presence and distribution of their receptors.

Objective: To evaluate the expression of type 2 TNF-α receptor (TNF-R2) in fragments of cholesteatoma and correlate it to the degree of inflammation present.

Material and methods: observational cross-sectional study, which analyzed 33 fragments of cholesteatomas through histological analysis and immunohistochemistry (using as primary antibody to TNF-R2 LabVision® brand). The evaluation was performed by means of a qualitative and semi-quantitative agreement with the observed intensity. For statistical analysis we used the Fisher exact test and Spearman's correlation coefficient (considered statistically significant when p < 0. 05).

Results: The expression of TNF-R2 was present in all fragments, however a statistical analysis showed no correlation or association between inflammation and the expression of TNF-R2.

Conclusions: TNF-R2 is present in cholesteatoma of the middle ear, however, its expression is not directly related to the degree of inflammation observed in patients with this disease.

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Figures

Figure 1
Figure 1
Microphotography from a cholesteatoma fragment, in which we see a weak reaction against the TNF-R2 receptor in the cytoplasm of the matrix keratinocytes (IHQ X400).
Figure 2
Figure 2
Microphotography of the cholesteatoma fragment, in which we notice moderate reaction against the TNF-R2 receptor in the cytoplasm of the matrix keratinocytes (IHQ X400).
Figure 3
Figure 3
Microphotography of the cholesteatoma fragment where we see a severe reaction against the TNF-R2 antibody in the cytoplasm of matrix keratinocytes (IHQ X400).
Figure 4
Figure 4
Inflammatory process severity analysis.
Figure 5
Figure 5
ITNR-R2 distribution in the cholesteatoma fragments analyzed.
See this image and copyright information in PMC

References

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