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Comparative Study
. 1990 Apr 11;18(7):1687-91.
doi: 10.1093/nar/18.7.1687.

DNA recombination during PCR

A Meyerhans  1 , J P Vartanian, S Wain-Hobson
Affiliations
Free PMC article
Comparative Study

DNA recombination during PCR

A Meyerhans et al. Nucleic Acids Res. .
Free PMC article

Abstract

PCR co-amplification of two distinct HIV1 tat gene sequences lead to the formation of recombinant DNA molecules. The frequency of such recombinants, up to 5.4% of all amplified molecules, could be decreased 2.7 fold by a 6 fold increase in Taq DNA polymerase elongation time. Crossover sites mapped essentially to three discrete regions suggesting specific Taq DNA polymerase pause or termination sites. PCR mediated recombination may be a problem when studying heterogeneous genetic material such as RNA viruses, multigene families, or repetitive sequences. This phenomenon can be exploited to create chimeric molecules from related sequences.

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References

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