Evaluation of the subgingival microbiota of alcoholic and non-alcoholic individuals

Abstract

Objectives: The aim of this study was to evaluate the composition of the subgingival microbiota of alcoholic and non-alcoholic individuals.

Methods: The study was conducted with 49 alcoholic and 49 non-alcoholic males of the Philippe Pinel Institute, Rio de Janeiro, Brazil. The subjects were selected by convenience and two criteria were used to diagnose alcohol dependence: the CAGE (cut-down, annoyed, guilt, eyes-opener) questionnaire and the International Statistical Classification of Diseases, 10th edition (WHO). Subgingival biofilm samples were obtained from 4 sites, 2 with probing depth (PD) ≥ 4 mm and 2 sites with PD < 4 mm. The presence and levels of 45 bacterial taxa were analysed using the checkerboard technique.

Results: The prevalence of bacterial species was not significantly different between groups. However, alcoholics showed significantly higher mean counts of Aggregactibacter actinomycetemcomitans, Fusobacterium nucleatum nucleatum, and Porphyromonas gingivalis (adjusted p < 0.001). Moreover, alcoholics harboured significantly higher mean levels of Capnocytophaga sputigena, Fusobacterium nucleatum vincentii, F. nuc. nucleatum, Gemella morbillorum, Neisseria mucosa, P. gingivalis, Streptococcus gordonii, and Tannerella forsythia at sites with PD < 4 mm or ≥ 4 mm compared to non-alcoholics (p ≤ 0.001). Of interest, shallow sites of alcoholics presented significantly higher mean levels of F. nuc. vincentii, F. nuc nucleatum, P. gingivalis, and T. forsythia than sites with PD ≥ 4 mm of non-alcoholics (p ≤ 0.001).

Conclusions: Alcoholics and non-alcoholics present a diverse and complex microbiota; however, alcoholics harbour significantly higher levels of periodontopathic species in the subgingival microbiota than non-alcoholics.