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Comparative Study
. 2011 Oct;49(10):3659-62.
doi: 10.1128/JCM.00211-11. Epub 2011 Aug 24.

Comparison of two nucleic acid amplification assays, the Xpert MTB/RIF assay and the amplified Mycobacterium Tuberculosis Direct assay, for detection of Mycobacterium tuberculosis in respiratory and nonrespiratory specimens

Jeanette Teo  1 , Roland Jureen, Donald Chiang, Douglas Chan, Raymond Lin
Affiliations
Comparative Study

Comparison of two nucleic acid amplification assays, the Xpert MTB/RIF assay and the amplified Mycobacterium Tuberculosis Direct assay, for detection of Mycobacterium tuberculosis in respiratory and nonrespiratory specimens

Jeanette Teo et al. J Clin Microbiol. 2011 Oct.

Abstract

We compared the performance of the Xpert MTB/RIF assay, a new real-time tuberculosis (TB) PCR test, with that of the Amplified Mycobacterium Tuberculosis Direct (MTD) assay using 162 respiratory and nonrespiratory specimens. Based on culture as the gold standard, the overall sensitivity and specificity for all sample types for the Xpert MTB/RIF assay were 90.9 and 89%, respectively, while for the MTD assay, the overall sensitivity and specificity were 97.3 and 87.1%, respectively. A higher proportion of total equivocal results were obtained for the MTD assay, at 10.5% (17/162), while the Xpert MTB/RIF assay generated 5.5% (9/162) of invalid reads.

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References

    1. Boehme C. C., et al. 2010. Rapid molecular detection of tuberculosis and rifampin resistance. N. Engl. J. Med. 363:1005–1015 - PMC - PubMed
    1. Coll P., Garrigó M., Moreno C., Martí N. 2003. Routine use of Gen-Probe Amplified Mycobacterium Tuberculosis Direct (MTD) test for detection of Mycobacterium tuberculosis with smear-positive and smear-negative specimens. Int. J. Tuberc. Lung Dis. 7:886–891 - PubMed
    1. Helb D., et al. 2010. Rapid detection of Mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology. J. Clin. Microbiol. 48:229–237 - PMC - PubMed
    1. Metchock B., Nolte F., Wallace R., Jr 2007. Mycobacterium. In Murray P. R., Baron E. J., Jorgensen J. H., Landry M. L., Pfaller M. A. (ed.), Manual of clinical microbiology. ASM Press, Washington, DC
    1. Moure R., et al. 2011. Rapid detection of Mycobacterium tuberculosis complex and rifampin resistance in smear-negative clinical samples using an integrated real time PCR method. J. Clin. Microbiol. 49:1137–1139 - PMC - PubMed

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