Salmonid pituitary gonadotrophs. II. Ontogeny of GTH I and GTH II cells in the rainbow trout (Salmo gairdneri irideus)

Abstract

Immunocytochemistry of rainbow trout pituitary gonadotrophs (GTH I- and GTH II-producing cells) during gametogenesis was investigated. GTH I and GTH II were found in distinctly different cells in all stages of reproductive development that were examined. Only GTH I cells were present in trout prior to puberty. GTH II appeared in addition to GTH I coincident with the onset of vitellogenesis and spermatogenesis. Both GTH I and GTH II cells were found in trout at the time of final reproductive maturation, although the number of GTH II cells was greater than that of GTH I cells. These data indicate that GTH I and GTH II are localized in separate cells in the trout pituitary throughout gametogenesis, and that synthesis of GTH I and GTH II varies during reproductive development.