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. 2011 Dec;13(12):912-8.
doi: 10.1016/j.jfms.2011.07.009. Epub 2011 Aug 31.

Detection of protective antibody titers against feline panleukopenia virus, feline herpesvirus-1, and feline calicivirus in shelter cats using a point-of-care ELISA

Brian A Digangi  1 , Lauren K Gray, Julie K Levy, Edward J Dubovi, Sylvia J Tucker
Affiliations

Detection of protective antibody titers against feline panleukopenia virus, feline herpesvirus-1, and feline calicivirus in shelter cats using a point-of-care ELISA

Brian A Digangi et al. J Feline Med Surg. 2011 Dec.

Abstract

Serum antibody titers are a useful measurement of protection against infection (feline panleukopenia virus [FPV]) or clinical disease (feline herpesvirus-1 [FHV] and feline calicivirus [FCV]), and their determination has been recommended as part of disease outbreak management in animal shelters. The objective of this study was to determine the sensitivity, specificity, and inter-observer and inter-assay agreement of two semi-quantitative point-of-care assays for the detection of protective antibody titers (PAT) against FPV, FHV and FCV in shelter cats. Low sensitivity for FPV antibodies (28%) rendered a canine point-of-care assay inappropriate for use in cats. The feline point-of-care assay also had low sensitivity (49%) and low negative predictive value (74%) for FPV PAT detection, but was highly accurate in the assessment of FHV and FCV PAT. Improvements in accuracy and repeatability of FPV PAT determination could make this tool a valuable component of a disease outbreak response in animal shelters.

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Conflict of interest statement

Support for this study was provided by the Sweetbay Foundation and Maddie's Fund.

Figures

Fig 1.
Fig 1.
A feline point-of-care ELISA kit for the determination of PAT against FPV, FHV, and FCV. A 12-toothed comb with antigen test spots is moved through wells of the developing plate at timed intervals (A). The results are displayed as gray color tones and are compared to a Comb Scale for interpretation (B).
See this image and copyright information in PMC

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