Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Dec;13(12):912-8.
doi: 10.1016/j.jfms.2011.07.009. Epub 2011 Aug 31.

Detection of protective antibody titers against feline panleukopenia virus, feline herpesvirus-1, and feline calicivirus in shelter cats using a point-of-care ELISA

Brian A Digangi  1 , Lauren K Gray, Julie K Levy, Edward J Dubovi, Sylvia J Tucker
Affiliations

Detection of protective antibody titers against feline panleukopenia virus, feline herpesvirus-1, and feline calicivirus in shelter cats using a point-of-care ELISA

Brian A Digangi et al. J Feline Med Surg. 2011 Dec.

Abstract

Serum antibody titers are a useful measurement of protection against infection (feline panleukopenia virus [FPV]) or clinical disease (feline herpesvirus-1 [FHV] and feline calicivirus [FCV]), and their determination has been recommended as part of disease outbreak management in animal shelters. The objective of this study was to determine the sensitivity, specificity, and inter-observer and inter-assay agreement of two semi-quantitative point-of-care assays for the detection of protective antibody titers (PAT) against FPV, FHV and FCV in shelter cats. Low sensitivity for FPV antibodies (28%) rendered a canine point-of-care assay inappropriate for use in cats. The feline point-of-care assay also had low sensitivity (49%) and low negative predictive value (74%) for FPV PAT detection, but was highly accurate in the assessment of FHV and FCV PAT. Improvements in accuracy and repeatability of FPV PAT determination could make this tool a valuable component of a disease outbreak response in animal shelters.

PubMed Disclaimer

Conflict of interest statement

Support for this study was provided by the Sweetbay Foundation and Maddie's Fund.

Figures

Fig 1.
Fig 1.
A feline point-of-care ELISA kit for the determination of PAT against FPV, FHV, and FCV. A 12-toothed comb with antigen test spots is moved through wells of the developing plate at timed intervals (A). The results are displayed as gray color tones and are compared to a Comb Scale for interpretation (B).
See this image and copyright information in PMC

References

    1. Patterson EV, Reese MJ, Tucker SJ, Dubovi EJ, Crawford PC, Levy JK. Effect of vaccination on parvovirus antigen testing in kittens. J Am Vet Med Assoc 2007; 230: 359–63. - PubMed
    1. Pappas ML. Panleukopenia fears. Nuvo Indy's alternative voicehttp://www.nuvo.net/indianapolis/panleukopeniafears/Content?oid=1229350; 2005. (accessed Dec 19, 2010).
    1. King R. Everett euthanizes cats, closes feline shelter to stem disease outbreak. Everett: HeraldNetcom; http://www.heraldnet.com/article/20100927/NEWS01/709279847; 2010. (accessed Dec 19, 2010).
    1. Dinnage JD, Scarlett JM, Richards JR. Descriptive epidemiology of feline upper respiratory tract disease in an animal shelter. J Feline Med Surg 2009; 11: 816–25. - PMC - PubMed
    1. Edinboro CH, Janowitz LK, Guptill-Yoran L, Glickman LT. A clinical trial of intranasal and subcutaneous vaccines to prevent upper respiratory infection in cats at an animal shelter. J Feline Med Surg Pract 1999; 27: 7–13.

Publication types

MeSH terms

Substances