Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Sep 1;118(9):2633-4.
doi: 10.1182/blood-2011-02-336479.

Confocal imaging studies cast doubt on nuclear localization of JAK2V617F

François Girodon, Mara P Steinkamp, Cédric Cleyrat, Sylvie Hermouet, Bridget S Wilson

Confocal imaging studies cast doubt on nuclear localization of JAK2V617F

François Girodon et al. Blood. .
No abstract available

PubMed Disclaimer

Figures

Figure 1
Figure 1
JAK2 targets the membrane of Baf3 and HEL cells and is excluded from the nucleus. (A) Confocal fluorescent images in Baf3/EPOR cells after transient transfection with JAK2WT-mCitrine. The nucleus is labeled with Hoechst. (B) Confocal images of Baf3/EpoR cells transiently transfected with JAK2V617F-mCitrine (green fluorescence). Cells were also stained with anti-JAK2 (Cy5, red fluorescence) and Hoechst. (C-D) Analysis of endogenous JAK2 distribution in HEL and K562 cells labeled with Hoechst and anti-JAK2 antibodies. Red lines indicate lines drawn across individual cells in the confocal image, with matching plots below reporting fluorescence intensity. (E) Analysis of nuclear and cytosol extracts from HEL cells, subjected to SDS-PAGE, Western blotting and immunoblotting with JAK2, tubulin, and phospho-histone 3 antibodies.(F-G) Confocal fluorescent images in HEL and K562 cells after transient (HEL) or stable (K562) transfection with JAK2V617F-mCitrine (HEL) or JAK2WT-mCitrine (K562) (green fluorescence). Nuclei were stained with Hoechst and endogenous JAK2 was stained with an anti-JAK2 antibody detected by a secondary antibody coupled to a Cy5 dye. Confocal fluorescent images were acquired using a Zeiss LSM 510 META confocal microscope with a 63× oil objective. ZEN 2009 5.5 SP1 software was used for both image acquisition and processing. Before imaging, treated cells were mounted on slides using ProLong Gold antifade reagent (Invitrogen).
See this image and copyright information in PMC

Comment in

  • Nuclear JAK2.
    Dawson MA, Bannister AJ, Saunders L, Wahab OA, Liu F, Nimer SD, Levine RL, Göttgens B, Kouzarides T, Green AR. Dawson MA, et al. Blood. 2011 Dec 22;118(26):6987-8. doi: 10.1182/blood-2011-10-385278. Blood. 2011. PMID: 22194397 Free PMC article. No abstract available.

References

    1. James C, Ugo V, Le Couedic JP, et al. A unique clonal JAK2 mutation leading to constitutive signalling causes polycythaemia vera. Nature. 2005;434(7037):1144–1148. - PubMed
    1. Rinaldi CR, Rinaldi P, Alagia A, et al. Preferential nuclear accumulation of JAK2V617F in CD34+ but not in granulocytic, megakaryocytic, or erythroid cells of patients with Philadelphia-negative myeloproliferative neoplasia. Blood. 2010;116(26):6023–6026. - PubMed
    1. Dawson MA, Bannister AJ, Gottgens B, et al. JAK2 phosphorylates histone H3Y41 and excludes HP1alpha from chromatin. Nature. 2009;461(7265):819–822. - PMC - PubMed
    1. Behrmann I, Smyczek T, Heinrich PC, et al. Janus kinase (Jak) subcellular localization revisited: the exclusive membrane localization of endogenous Janus kinase 1 by cytokine receptor interaction uncovers the Jak.receptor complex to be equivalent to a receptor tyrosine kinase. J Biol Chem. 2004;279(34):35486–35493. - PubMed
    1. Lobie PE, Ronsin B, Silvennoinen O, Haldosen LA, Norstedt G, Morel G. Constitutive nuclear localization of Janus kinases 1 and 2. Endocrinology. 1996;137(9):4037–4045. - PubMed

Publication types

MeSH terms