Efficacy of different disinfectant systems on alginate and addition silicone impression materials of Indian and international origin: a comparative evaluation

Abstract

Study was planned to evaluate the efficacy of commonly used disinfectants and to study qualitatively and quantitatively the persistence of microflora on the untreated (control group) and the disinfected impression surface after 24 h. Disinfectant systems used were immersion systems like glutaraldehyde, sodium hypochlorite and the ultraviolet chamber. The effect of disinfectant on most commonly used Indian impression materials was carried out in this study and results compared with the most commonly used foreign brands for irreversible hydrocolloid and addition silicone. Impressions were made of 25 healthy volunteers. These were disinfected and incubated in an incubator for 24 h at 37°C for aerobic organisms. The inoculation in nutrient media was done to test the viability of microorganisms that can persist after rinsing and disinfection of the impression surface. The colony forming units were counted and compared with that of control group. Control group of all the impression material samples showed growth of Streptococcus viridans, Diphtheroids, Streptococcus pneumoniae to a greater extent. The growth of Candida albicans, Pseudomonas aerugenosa and Staphyloccus albus was present in all the groups but to a lesser extent. The persistence of the microflora on the impression surface of both the studied brands was similar but the concentration of organisms in the alginate control group was two folds as compared to addition silicone group. Use of ultraviolet chamber gave better results compared to the studied immersion systems. All the disinfection systems were effective in reducing the microbial load with ultraviolet chamber as the most effective.

Keywords: Disinfection; Immersion systems; Ultraviolet chamber.

Fig. 1

Immersion system & control group

Fig. 2

UV Light disinfection unit

Fig. 3

Comparison of microbial flora with different disinfectant systems on Algin- Gum alginate sample

Fig. 4

Comparison of microbial flora with different disinfectant systems on Vignette alginate sample

Fig. 5

Comparison of microbial flora with different disinfectant systems on Ad-Sil Addition silicone sample

Fig. 6

Comparison of microbial flora with different disinfectants on Aquasil Addition silicone sample