Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Sep;12(9):760-8.
doi: 10.1631/jzus.B1000440.

Changes in bacterial community of anthracene bioremediation in municipal solid waste composting soil

Shu-ying Zhang  1 , Qing-feng Wang, Rui Wan, Shu-guang Xie
Affiliations

Changes in bacterial community of anthracene bioremediation in municipal solid waste composting soil

Shu-ying Zhang et al. J Zhejiang Univ Sci B. 2011 Sep.

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are common contaminants in a municipal solid waste (MSW) composting site. Knowledge of changes in microbial structure is useful to identify particular PAH degraders. However, the microbial community in the MSW composting soil and its change associated with prolonged exposure to PAHs and subsequent biodegradation remain largely unknown. In this study, anthracene was selected as a model compound. The bacterial community structure was investigated using terminal restriction fragment length polymorphism (TRFLP) and 16S rRNA gene clone library analysis. The two bimolecular tools revealed a large shift of bacterial community structure after anthracene amendment and subsequent biodegradation. Genera Methylophilus, Mesorhizobium, and Terrimonas had potential links to anthracene biodegradation, suggesting a consortium playing an active role.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Terminal restriction fragments (HaeIII digest) and their abundances in the Samples A and B
Fig. 2
Fig. 2
Percentages of the clones affiliated with different phyla and sub-phyla to the total number of clones from Sample A or B Clones not classified to any known phylum are included as unclassified bacteria
Fig. 3
Fig. 3
Phylogenetic tree of representative bacterial 16S rRNA gene sequences (beginning with ‘L’) within the genera of Methylophilus, Mesorhizobium, and Terrimonas from Sample B and reference sequences from GenBank Data in parentheses are GenBank accession numbers. Numbers at the nodes indicate the levels of bootstrap support based on neighbor-joining analysis of 1 000 resampled datasets
See this image and copyright information in PMC

References

    1. Abed RMM, Safi NMD, Köster J, de Beer D, El-Nahhal Y, Rullkötter J, Garcia-Pichel F. Microbial diversity of a heavily polluted microbial mat and its community changes following degradation of petroleum compounds. Appl Environ Microbiol. 2002;68(4):1674–1683. doi: 10.1128/AEM.68.4.1674-1683.2002. - DOI - PMC - PubMed
    1. Baun A, Ledin A, Reitzel LA, Bjerg PL, Christensen TH. Xenobiotic organic compounds in leachates from ten Danish MSW landfills—chemical analysis and toxicity tests. Water Res. 2004;38(18):3845–3858. doi: 10.1016/j.watres.2004.07.006. - DOI - PubMed
    1. Cébron A, Beguiristain T, Faure P, Norini MP, Masfaraud JF, Leyval C. Influence of vegetation on the in situ bacterial community and polycyclic aromatic hydrocarbon degraders in aged PAH-contaminated or thermal-desorption-treated soil. Appl Environ Microbiol. 2009;75(19):6322–6330. doi: 10.1128/AEM.02862-08. - DOI - PMC - PubMed
    1. Chang YT, Lee JF, Chao HP. Variability of communities and physiological characteristics between free-living bacteria and attached bacteria during the PAH biodegradation in a soil/water system. Eur J Soil Biol. 2007;43(5-6):283–296. doi: 10.1016/j.ejsobi.2007.02.012. - DOI
    1. Chen WM, Zhu WF, Bontemps C, Young JPW, Wei GH. Mesorhizobium alhagi sp. nov. isolated from wild Alhagi sparsifolia in north-western China. Int J Syst Evol Microbiol. 2010;60(4):958–962. doi: 10.1099/ijs.0.014043-0. - DOI - PubMed

Publication types