[Comparative investigation by spectrofluorimetry and flow cytometry of plasma and inner mitochondrial membranes polarisation in smooth muscle cell using potential-sensitive probe DiOC6(3)]

Abstract

Possibility of the use of flow cytometry and spectrofluorimetry analysis for investigation mitochondria and plasma membrane polarization in myometrium cell suspension using potential-sensitive probe 3,3'-dihexyloxacarbocyanine [DiOC6(3)] has been demonstrated. The obtained results confirm the use of DiOC6(3) for studying the influence of effectors on transmembrane potentials of intact cell compartments.