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. 2011 Nov;193(21):6070-4.
doi: 10.1128/JB.05523-11. Epub 2011 Sep 2.

Mutations in genes patA and patL of Anabaena sp. strain PCC 7120 result in similar phenotypes, and the proteins encoded by those genes may interact

Jinjie Liu  1 , C Peter Wolk
Affiliations

Mutations in genes patA and patL of Anabaena sp. strain PCC 7120 result in similar phenotypes, and the proteins encoded by those genes may interact

Jinjie Liu et al. J Bacteriol. 2011 Nov.

Abstract

PatA resembles a response regulator protein with a defective DNA-binding domain, and PatL (All3305) is a pentapeptide repeat protein. A yeast two-hybrid library identified PatL as a protein with which PatA may interact. Heterocysts of patA and patL Anabaena sp. form nearly exclusively terminally in long filaments, further linking the genes.

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Figures

Fig. 1.
Fig. 1.
(a) Wild-type Anabaena sp. has semiregularly spaced heterocysts. (b) In contrast, the all3305 (patL) mutant DR3304b forms heterocysts almost exclusively at the end(s) of the filaments, a phenotype resembling that of a patA mutant (29). Heterocysts are stained with Alcian blue.
Fig. 2.
Fig. 2.
Pentapeptide repeat region of All3305. The 45 pentapeptide repeats of the 496-amino-acid predicted protein All3305 near its C terminus are largely [A35/C4/V4][N24/D6/K5] [L37/F5][X][G20/X]. Amino acid numbers are shown.
See this image and copyright information in PMC

References

    1. Altschul S. F., et al. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389–3402 - PMC - PubMed
    1. Becker D. M., Guarente L. 1991. High-efficiency transformation of yeast by electroporation. Methods Enzymol. 194:182–187 - PubMed
    1. Black K., Buikema W. J., Haselkorn R. 1995. The hglK gene is required for localization of heterocyst-specific glycolipids in the cyanobacterium Anabaena sp. strain PCC 7120. J. Bacteriol. 177:6440–6448 - PMC - PubMed
    1. Black T. A., Cai Y., Wolk C. P. 1993. Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena. Mol. Microbiol. 9:77–84 - PubMed
    1. Black T. A., Wolk C. P. 1994. Analysis of a Het− mutation in Anabaena sp. strain PCC 7120 implicates a secondary metabolite in the regulation of heterocyst spacing. J. Bacteriol. 176:2282–2292 - PMC - PubMed

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