Tightly controlled expression systems for the production and purification of Escherichia coli release factor 1
Affiliations
- PMID: 2189411
Item in Clipboard
Tightly controlled expression systems for the production and purification of Escherichia coli release factor 1
Biochem Int.
1990.
Abstract
The genes for the protein release factors in Escherichia coli have traditionally proven difficult to maintain on high copy plasmids. We have established here systems which provide for both stable maintenance of the release factor 1 gene on such plasmids, as well as high level overproduction of the release factor 1 protein. The gene is maintained under the control of the inducible trc or tac promoters in the presence of very high levels of lac repressor. A simple and rapid scheme for the purification of RF1 from extracts of cultures carrying these plasmids is also described.
Similar articles
-
Control of acetate production rate in Escherichia coli by regulating expression of single-copy pta using lacI(Q) in multicopy plasmid.J Microbiol Biotechnol. 2008 Feb;18(2):334-7. J Microbiol Biotechnol. 2008. PMID: 18309280
-
Production of soluble and active recombinant murine interleukin-2 in Escherichia coli: high level expression, Kil-induced release, and purification.Protein Expr Purif. 1995 Aug;6(4):481-6. doi: 10.1006/prep.1995.1064. Protein Expr Purif. 1995. PMID: 8527934
-
Effects of release factor 1 on in vitro protein translation and the elaboration of proteins containing unnatural amino acids.Biochemistry. 1999 Jul 6;38(27):8808-19. doi: 10.1021/bi990281r. Biochemistry. 1999. PMID: 10393557
-
Expression of peptide chain release factor 2 requires high-efficiency frameshift.Nature. 1986 Jul 17-23;322(6076):273-5. doi: 10.1038/322273a0. Nature. 1986. PMID: 3736654
-
Tightly regulated, high-level expression from controlled copy number vectors based on the replicon of temperate phage N15.Gene. 2007 Jun 15;395(1-2):15-21. doi: 10.1016/j.gene.2006.12.036. Epub 2007 Jan 20. Gene. 2007. PMID: 17433573
Cited by
-
Expression of mitochondrial release factor in relation to respiratory competence in yeast.Curr Genet. 2005 Aug;48(2):101-8. doi: 10.1007/s00294-005-0582-4. Epub 2005 Sep 14. Curr Genet. 2005. PMID: 16021449
-
Identification of putative programmed -1 ribosomal frameshift signals in large DNA databases.Genome Res. 1999 May;9(5):417-27. Genome Res. 1999. PMID: 10330121 Free PMC article.
-
Modulation of HIV-1 Gag/Gag-Pol frameshifting by tRNA abundance.Nucleic Acids Res. 2019 Jun 4;47(10):5210-5222. doi: 10.1093/nar/gkz202. Nucleic Acids Res. 2019. PMID: 30968122 Free PMC article.
-
Frameshift autoregulation in the gene for Escherichia coli release factor 2: partly functional mutants result in frameshift enhancement.Nucleic Acids Res. 1990 Nov 25;18(22):6517-22. doi: 10.1093/nar/18.22.6517. Nucleic Acids Res. 1990. PMID: 2251114 Free PMC article.
-
Inhibition of the release factor-dependent termination reaction on ribosomes by DnaJ and the N-terminal peptide of rhodanese.J Bacteriol. 1995 Oct;177(19):5517-22. doi: 10.1128/jb.177.19.5517-5522.1995. J Bacteriol. 1995. PMID: 7559337 Free PMC article.