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. 2011 Dec;21(12):1750-4.
doi: 10.1038/cr.2011.151. Epub 2011 Sep 6.

Argonaute-2 regulates the proliferation of adult stem cells in planarian

Yong-Qin Li, An Zeng, Xiao-Shuai Han, Chen Wang, Ge Li, Zhen-Chao Zhang, Jian-Yong Wang, Yong-Wen Qin, Qing Jing

Argonaute-2 regulates the proliferation of adult stem cells in planarian

Yong-Qin Li et al. Cell Res. 2011 Dec.

Erratum in

  • Cell Res. 2012 Jan;22(1):275
No abstract available

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Figures

Figure 1
Figure 1
Argonaute-2 regulates the proliferation of adult stem cells in planarian. (A) Regeneration defects after knockdown of Ago2. Worms were amputated at 9 daf. Each panel shows a dorsal view of trunk fragments regenerating a new head and tail, with anterior at the left. Scale bar: 250 μm. (B) Tissue regression of intact Ago2(RNAi) animals. Each panel shows a dorsal view. Scale bar: 250 μm. (C) Survival curve. Percentage of live animals after control, Ago1and Ago2 RNAi, n = 50. (D) qRT-PCR showing the relative expression level of stem cell markers in planarians at 15 daf. (E) Analysis of the expression of stem cell markers at 10 daf by WISH. Smedwi-1 and Smed-PCNAwere used as stem cell markers. Scale bar: 50 μm. (F) Immunofluorescence for SMEDWI-1 in amputated worms at 14, 43, 96 and 144 h after amputation. Each panel shows a dorsal view of tail fragments regenerating a new head, with anterior at the bottom. All images are single confocal sections. Scale bar: 50 μm. (G) Numbers of cells labeled with an anti-Brdu antibody were divided by animal surface area. All worms were examined at 15 daf. (H) Numbers of mitoses labeled with an anti-phospho histone 3 (H3P) antibody were divided by animal surface area. All worms were examined at 15 daf. (I) Numbers of mitoses labeled with anti-H3-S10P were divided by animal surface area. Animals were fed three times and amputated at 1 day after the last feeding, then animals were fixed at various time points after amputation. (J) qRT-PCR showing the relative expression of several miRNAs in planarians at 15 daf. (D, G-J) Data are means ± sd (n = 3 experiments, > 4 worms per time point). **P < 0.001, ***P < 0.0001, Student's t-test.
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