Evaluation of antioxidant potential of Amalakayas Rasayana: A polyherbal Ayurvedic formulation

Abstract

Amalakayas Rasayana (AR) is a polyherbal formulation mentioned in Ayurveda to treat aging and age-associated diseases. Being an antiaging drug, AR may have antioxidants and free radical scavenging activity to minimize free radical-induced damage which is a key cause of aging. The methanolic extract of AR was evaluated in vitro for total phenolic and tannin content, free radical scavenging activity, superoxide radical scavenging activity, and reducing power. The total phenolic content was measured using Folin-ciocalteu reagent against gallic acid [relative standard deviation (R(2)) = 0.998]. Total tannin was estimated using the Stephen method and was found to be 2.82% w/w. Free radical scavenging activity was measured by 2,2-diphenyl-1-picryl hydrazyl assay and R(2) was 1. Superoxide radical scavenging activity was done by ethylene diamine tetra acetate and Nitro Blue Tetrazolium Chloride assays against ascorbic acid and R(2) was 0.976 (EC(50)= 77.5 μg/ml). Ferrous reducing power was evaluated by Oyaizu method where R(2) was 0.986. All studies showed that AR possesses antioxidant activity. The results of this study suggest that the antioxidant and free radical scavenging activity of AR may explain its rasayana effect and justify its use as a medicine for age associated diseases.

Keywords: Amalakayas Rasayana; Antioxidant; DPPH; anti-radical; reducing power.

Figure 1

Absorbance (at 765 nm) of gallic acid and methanol extract of AR in different concentrations (μg/ml)

Figure 2

Free radical scavenging activity, % inhibition versus concentration for standard ascorbic acid (AA) and AR (polynomial plotting of second degree)

Figure 3a

DPPH assay—polynomial plotting of third degree of Ascorbic acid

Figure 3b

DPPH assay—polynomial plotting of third degreeof AR

Figure 4

Superoxide radical scavenging activity, % inhibition versus concentration for standard ascorbic acid and test drug AR

Figure 5

Reducing power of methanol extract of AR