Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011:787:255-65.
doi: 10.1007/978-1-61779-295-3_19.

Preparation of a heat-shock protein 70-based vaccine from DC-tumor fusion cells

Desheng Weng  1 , Stuart K Calderwood, Jianlin Gong
Affiliations

Preparation of a heat-shock protein 70-based vaccine from DC-tumor fusion cells

Desheng Weng et al. Methods Mol Biol. 2011.

Abstract

We have developed an enhanced molecular chaperone-based vaccine through rapid isolation of heat-shock protein 70 peptide complexes (Hsp70.PC) after the fusion of tumor and dendritic cells (DCs) (Hsp70.PC-F). In this approach, the tumor antigens are introduced into the antigen-processing machinery of dendritic cells through the cell fusion process and, thus, we can obtain antigenic tumor peptides or their intermediates that have been processed by dendritic cells. Our results show that Hsp70.PC-F has increased immunogenicity compared to preparations from tumor cells alone and, therefore, constitutes an improved formulation of chaperone protein-based tumor vaccine.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Flow chart for HSP70.PC purification
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Lindquist S, Craig EA. The heat shock proteins. Ann. Rev. Genet. 1988;22:631–637. - PubMed
    1. Georgopolis C, Welch WJ. Role of the major heat shock proteins as molecular chaperones. Ann. Rev. Cell Biol. 1993;9:601–634. - PubMed
    1. Bukau B, Horwich AL. The Hsp70 and Hsp60 chaperone machines. Cell. 1998;92:351–366. - PubMed
    1. Tang D, Khaleque AA, Jones ER, Theriault JR, Li C, Wong WH, Stevenson MA, et al. Expression of heat shock proteins and HSP messenger ribonucleic acid in human prostate carcinoma in vitro and in tumors in vivo. Cell Stress Chaperones. 2005;10 in press. - PMC - PubMed
    1. Noessner E, Gastpar R, Milani V, Brandl A, Hutzler PJ, Kuppner MC, Roos M, et al. Tumor-derived heat shock protein 70 peptide complexes are cross-presented by human dendritic cells. J Immunol. 2002;169:5424–5432. - PubMed

MeSH terms

LinkOut - more resources