Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007:2:221-8.
doi: 10.4137/dti.s220. Epub 2007 Sep 18.

Effect of live Salmonella Ty21a in dextran sulfate sodium-induced colitis

Gunnar Nysœter  1 , Kari Erichsen, Anne Marita Milde, Eva Colás, Einar Kristoffersen, Arnold Berstad
Affiliations

Effect of live Salmonella Ty21a in dextran sulfate sodium-induced colitis

Gunnar Nysœter et al. Drug Target Insights. 2007.

Abstract

Background: Intestinal microbiota seems to play an essential role in the development of inflammatory bowel diseases (IBD). We hypothesised that an oral vaccine based on live Salmonella typhi would be well tolerated and could even attenuate dextran sulfate sodium (DSS) induced colitis in rats, an animal model of IBD.

Methods: Nine male Wistar rats was used for an initial tolerance study, in which we used 3 dose-levels of Salmonella Ty21a, 0.5 × 10(9), 1 × 10(9), and 2 × 10(9)CFU, each dose being tested in 3 rats. Four treatment groups consisting of 8 male Wistar rats per group: 1) control group given standard food and water, 2) control group given four daily administrations of Salmonella Ty21a 1 × 10(9) CFU, 3) water with 5% DSS the last 7 days, 4) four daily administrations of Salmonella Ty21a before water with 5% DSS the last 7 days. The Salmonella Ty21a was administered by gastric gavage on day 1, 3, 5 and 16, while DSS was given with the drinking water from day 15 to 22. The animals were sacrificed and colonic tissue removed for analysis 22 days after gavage of the first vaccine dose.

Results: The animals in the tolerance study got no signs of disease. In the treatment study, all animals receiving DSS had histologic indications of colitis, particularly in the distal part of the colon. Administration of Salmonella Ty21a had no significant effect on crypt and inflammation scores (p > 0.05).

Conclusion: Gastric administration of live vaccine strain Salmonella Ty21a was well tolerated, but did not provide any significant protection against development of DSS induced colitis in rats.

Keywords: colitis; inflammatory bowel disease; rats; salmonella Ty21a.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Weight gain from day 14–22 (DSS-period).
Figure 2
Figure 2
Salmonella Ty21a does not affect the level of granulocyte marker protein (GMP) in rats.
Figure 3
Figure 3
H&E × 20. Histologic normal mucosa in healthy control rat (a) and healthy Ty21a rat (b). Mucosa from DSS-group (c) and DSS-Ty21a-group (d) with severe inflammatory changes with crypt loss, crypt abscess and infiltration with inflammatory cells.
Figure 4
Figure 4
Histologic findings (crypt score and inflammatory score) in dextran sulphate induced colitis in rats is not significantly influenced by treatment with Salmonella Ty21a.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Bibiloni R, Fedorak RN, Tannock GW, Madsen KL, Gionchetti P, Campieri M, De Simone C, Sartor RB. VSL#3 probiotic-mixture induces remission in patients with active ulcerative colitis. Am. J. Gastroenterol. 2005;100(7):1539–46. - PubMed
    1. Boirivant M, Fuss IJ, Ferroni L, De Pascale M, Strober W. Oral administration of recombinant cholera toxin subunit B inhibits IL-12-mediated murine experimental (trinitrobenzene sulfonic acid) colitis. J. Immunol. 2001;166(5):3522–32. - PubMed
    1. Carrier J, Aghdassi E, Platt I, Cullen J, Allard JP. Effect of oral iron supplementation on oxidative stress and colonic inflammation in rats with induced colitis. Aliment. Pharmacol. Ther. 2001;15(12):1989–99. - PubMed
    1. Di Giacinto C, Marinaro M, Sanchez M, Strober W, Boirivant M. Probiotics ameliorate recurrent Th1-mediated murine colitis by inducing IL-10 and IL-10-dependent TGF-beta-bearing regulatory cells. J. Immunol. 2005;174(6):3237–46. - PubMed
    1. Engels EA, Falagas ME, Lau J, Bennish ML. Typhoid fever vaccines: a meta-analysis of studies on efficacy and toxicity. BMJ. 1998;316(7125):110–6. - PMC - PubMed