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. 2012:2012:261736.
doi: 10.1155/2012/261736. Epub 2011 Sep 8.

Behaviour of human erythrocyte aggregation in presence of autologous lipoproteins

C Saldanha  1 , J Loureiro, C Moreira, J Martins E Silva
Affiliations

Behaviour of human erythrocyte aggregation in presence of autologous lipoproteins

C Saldanha et al. Biochem Res Int. 2012.

Abstract

The aim of this work was to evaluate in vitro the effect of autologous plasma lipoprotein subfractions on erythrocyte tendency to aggregate. Aliquots of human blood samples were enriched or not (control) with their own HDL-C, LDL-C, or VLDL-C fractions obtained from the same batch by density gradient ultracentrifugation. Plasma osmolality and erythrocyte aggregation index (EAI) were determined. Blood aliquots enriched with LDL-C and HDL-C showed significant higher EAI than untreated aliquots, whereas enrichment with VLDL-C does not induce significant EAI changes. For the same range of lipoprotein concentrations expressed as percentage of osmolality variation, the EAI variation was positive and higher in presence of HDL-C than upon enrichment with LDL-C (P < 0.01). Particle size, up to LDL diameter values, seems to reinforce erythrocyte tendency to aggregate at the same plasma osmolality (particle number) range of values.

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Figures

Figure 1
Figure 1
Histogram presenting the concentrations of enriched blood samples with LDL-C (red), HDL-C (blue), VLDL-C (green) distributed by four classes according the osmolality scale of values (class I 0.005–0.025; class II 0.030–0.035: class III 0.045–0.055; class IV 0.077–0.095).
Figure 2
Figure 2
Histogram presenting the values of the erythrocyte aggregation variation obtained in enriched blood samples with LDL-C (red), HDL-C (blue), VLDL-C (green) in the four classes of concentration according the osmolality scale of values (I 0.005–0.025; II 0.030–0.035: III 0.045–0.055; IV 0.077–0.095).
Figure 3
Figure 3
Values of erythrocyte aggregation index (mean +/− sd) obtained in enriched blood samples with HDL-C and without enrichment.
Figure 4
Figure 4
Values of erythrocyte aggregation index (mean +/− sd) obtained in enriched blood samples with HDL-C and without enrichment
Figure 5
Figure 5
Values of erythrocyte aggregation index (mean +/− sd) obtained in enriched blood samples with LDL-C and without enrichment.
Figure 6
Figure 6
Presentation of the association obtained between the erythrocyte aggregation values and the osmolality values in enriched blood sample with HDL-C.
See this image and copyright information in PMC

References

    1. Kannel WB, Castelli WP, Gordon T. Cholesterol in the prediction of atherosclerotic disease: new perspectives based on the Framingham study. Annals of Internal Medicine. 1979;90(1):85–91. - PubMed
    1. Miller NE, Hammett F, Saltissi S, Vanzeller H, Coltart S, Lewis B. Relation of angiographically defined coronary artery disease to plasma lipoprotein subfractions and apolipoproteins. The British Medical Journal. 1981;282(6278):1741–1744. - PMC - PubMed
    1. Alshehri AM. Metabolic syndrome and cardiovascular risk. Journal of Family and Community Medicine. 2010;17:73–78. - PMC - PubMed
    1. Baigent C, Keech A, Kearney PM, Blackwell L, Buck G. Efficacy and safety of cholesterol-lowering treatment prospective meta-analysis of data from 90,056 participants in 14 randomised trials of statins. The Lancet. 2005;366:1267–1278. - PubMed
    1. Robinson JG, Smith B, Maheshwari N, Schrott H. Pleiotropic effects of statins: benefit beyond cholesterol reduction? A meta-regression analysis. Journal of the American College of Cardiology. 2005;46(10):1855–1862. - PubMed

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