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. 2011:791:23-32.
doi: 10.1007/978-1-61779-316-5_3.

Methylation-specific PCR

Ja-Lok Ku  1 , You-Kyung Jeon, Jae-Gahb Park
Affiliations

Methylation-specific PCR

Ja-Lok Ku et al. Methods Mol Biol. 2011.

Abstract

DNA methylation patterns in CpG-rich regions of promoter, CpG islands, are concerned in regulation of gene expression in mammalian cells. Excessive methylation of CpG dinucleotides in promoter represses the gene expression. In cancer, especially, gene silencing is occurred through aberrant methylation in promoter of tumor suppressor genes. Methylation-specific PCR (MSP) is a method for analysis of DNA methylation patterns in CpG islands. For performing MSP, DNA is modified by and PCR performed with two primer pairs, which are detectable methylated and unmethylated DNA, respectively. MSP is a rapid measure for assession of the methylation status in CpG island.

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