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Comment
. 2011 Sep 27;108(39):16137-8.
doi: 10.1073/pnas.1112842108. Epub 2011 Sep 13.

Neural circuits look forward

Sebnem N Tuncdemir  1 , Gord Fishell
Affiliations
Comment

Neural circuits look forward

Sebnem N Tuncdemir et al. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1.
Fig. 1.
Schematic description of the transneuronal tracers generated by Beier et al. (3) in PNAS. (A) The VSV genome was modified such that a fluorescent reporter (YFP) was placed upstream of viral proteins: nucleoprotein (N), phosphoprotein (P), matrix protein (M), G, and polymerase (L). VSV with endogenous G did not display transsynaptic spread. (B) Directionally selective polysynaptic tracers were generated by replacing the VSV-G with LCMV-G (purple) for anterograde spread, or with RV-G (orange) for retrograde spread of the viral vector. (C) G protein-deficient VSV (VSVΔG) pseudotyped with LCMV-G exhibited monosynaptic anterograde spread. (D) ASLV-A/RV-G pseudotyped VSVΔG can only infect the neurons expressing the TVA receptor from biolistic transfection (blue). By cotransfecting the gene for the LCMV-G in the same neurons, the VSVΔG can produce viruses that were able to transsynaptically infect postsynaptic neurons (Upper). VSVΔG repackaged with the G coat protein expressed from RV-G plasmid (orange) displayed transsynaptic spread to the presynaptic population of neurons (Lower). Because only the initially infected neuron contains virus G protein (LCMV-G or RV-G), VSV cannot spread any further, limiting VSV infection to neurons monosynaptically connected to initially infected population. A plasmid encoding mCherry was cotransfected for the cell originally targeted for infection to be identified within the monosynaptic network of GFP-labeled cells.
See this image and copyright information in PMC

Comment on

References

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