[Comparison of various diagnostic methods of Helicobacter infection]

Abstract

Purpose of investigation: There has been conducted comparison of the most wide-spread methods of diagnosis of Helicobacter pylori infection: histologic, cytologic, method of polymerase chain reaction (PMCR) and respiratory urease Xelic-test on the method of AMA firm.

Materials and methods: 1118 patients participated in the investigation, 403 patients were investigated by urease test and 50 ones were investigated by method of PMCR. The results of investigations were compared by means of correlative and variational analysis.

Results: There has been shown fine comprison of histologic, cytologic methods of Helicobacter pylori revealing and PMCR method both between them (coefficients of correlation 0.79, 0.34 and 0.53 accordingly), and with indices of gastritis activity (coefficients of correlation 0.49, 0.52 and 0.37 accordingly). On the other hand the results of respiratory urease Xelic-test did not correspond to any of investigated indices (coefficients of correlation 0.02, 0.05 and 0.09). The possible causes of such discrepancy, based on features of chemical properties of ammonia have been defined. Except known reaction of neutralization with hydrochloric acid, ammonia able to show donor-acceptor interactions and form various compounds with organic substances.

Conclusion: Urease respiratory Xelic-test on the method of AMA firm can not be used for definition of Helicobacter pylori infection.