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. 2011 Oct;136(4):625-30.
doi: 10.1309/AJCPKUM9J4IXCWEU.

Characteristic CD103 and CD123 expression pattern defines hairy cell leukemia: usefulness of CD123 and CD103 in the diagnosis of mature B-cell lymphoproliferative disorders

Girish Venkataraman  1 , Christine Aguhar, Robert J Kreitman, Constance M Yuan, Maryalice Stetler-Stevenson
Affiliations

Characteristic CD103 and CD123 expression pattern defines hairy cell leukemia: usefulness of CD123 and CD103 in the diagnosis of mature B-cell lymphoproliferative disorders

Girish Venkataraman et al. Am J Clin Pathol. 2011 Oct.

Abstract

By using flow cytometry, we studied CD103 and CD123 expression by the malignant cells in 300 B-cell lymphoproliferative disorder (BC-LPD) cases, including 114 hairy cell leukemia (HCL), 20 HCL variant (HCLv), 9 splenic marginal zone lymphoma (SMZL; in 5, only CD103 was evaluated), 133 chronic lymphocytic leukemia (CLL), 3 follicular lymphoma (FL), and 21 mantle cell lymphoma (MCL). All HCLs expressed uniform CD103 and bright CD123. Among the 20 HCLv cases, 20 (100%) were CD103+ and 8 (40%) were CD123+ (partial or dim). CD103 was negative in all MCL, FL, CLL, and SMZL cases. CD123 was positive in 1 (25%) of 4 SMZL, 3.8% of CLL (5/133), 7 (33%) of 21 MCL, and 1 (33%) of 3 FL cases. CD103 is specific for HCL and HCLv. CD123 expression is more widespread in BC-LPDs but is useful in conjunction with CD25 to differentiate HCLv from HCL. These findings support the usefulness of CD123 and CD103 to aid in the differential diagnosis of BC-LPDs.

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Conflict of interest statement

The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose.

Figures

Image 1
Image 1
Flow cytometric immunophenotypic detection of CD123 expression. A, Chronic lymphocytic leukemia (CLL) case negative for CD123. Analysis gate was drawn around the CD19+ and CD5+ CLL cells. B, CLL case with partial moderate CD123. Analysis gate was drawn around the CD19+ and CD5+ CLL cells. C, Mantle cell lymphoma (MCL) case with partial dim CD123. Analysis gate was drawn around the CD19+ and CD5+ MCL cells. D, Follicular lymphoma (FL) case with partial dim CD123. Analysis gate was drawn around the CD19+ and CD10+ FL cells. E, Splenic marginal zone lymphoma (SMZL) case with partial dim CD123. Analysis gate was drawn around the CD19+ SMZL cells (all B cells monoclonal). F, Hairy cell leukemia (HCL) case with uniform bright CD123. Analysis gate was drawn around the bright CD20+ and CD11c+ HCL cells. G, HCL-variant (HCLv) case with dim CD123. Analysis gate was drawn around the bright CD20+ and CD11c+ HCLv cells. FITC, fluorescein isothiocyanate; PE, phycoerythrin.
See this image and copyright information in PMC

References

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