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Comparative Study
. 2011 Nov;49(11):3943-6.
doi: 10.1128/JCM.00335-11. Epub 2011 Sep 14.

Evaluation of four commercial real-time PCR assays for detection of Bordetella spp. in nasopharyngeal aspirates

Philippe Lanotte  1 , Chloé Plouzeau, Christophe Burucoa, Carole Grélaud, Sophie Guillot, Nicole Guiso, Fabien Garnier
Affiliations
Comparative Study

Evaluation of four commercial real-time PCR assays for detection of Bordetella spp. in nasopharyngeal aspirates

Philippe Lanotte et al. J Clin Microbiol. 2011 Nov.

Abstract

We evaluated the performances of 4 commercial real-time PCR kits for Bordetella pertussis IS481 sequence detection in nasopharyngeal aspirates by comparison with an in-house real-time PCR assay. Among them, the Simplexa Bordetella pertussis/parapertussis assay (Focus Diagnostics), the SmartCycler Bordetella pertussis/parapertussis assay (Cepheid), and Bordetella R-gene (Argene) present sensitivities over 90%. One kit proved unsuitable for routine clinical use.

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Figures

Fig. 1.
Fig. 1.
Representation of the crossing threshold obtained for each commercial kit and the in-house real-time PCR for the detection of the Bordetella IS481 sequence.
See this image and copyright information in PMC

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