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. 1990 Jul;10(7):3828-33.
doi: 10.1128/mcb.10.7.3828-3833.1990.

Induction of transformation and DNA synthesis after microinjection of raf proteins

M R Smith  1 , G Heidecker, U R Rapp, H F Kung
Affiliations

Induction of transformation and DNA synthesis after microinjection of raf proteins

M R Smith et al. Mol Cell Biol. 1990 Jul.

Abstract

Full-length and N-terminal deletion mutants of human c-raf-1 cDNA were cloned into Escherichia coli expression plasmids. Bacterially expressed c-raf proteins were purified by anion-exchange, gel filtration, and affinity chromatography. Microinjection of mutant c-raf proteins into G0-arrested NIH 3T3 cells induced DNA synthesis and morphological transformation, whereas microinjection of full-length c-raf had no effect. The amino terminus of the raf protein has an important negative regulatory influence; alteration of this region resulted in increased kinase activity and oncogenicity.

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References

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