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Comment
. 2011 Sep 23;333(6050):1713-4.
doi: 10.1126/science.1212449.

Microbiology. Antibiotic resistance, not shaken or stirred

Ryan L Frisch  1 , Susan M Rosenberg
Affiliations
Comment

Microbiology. Antibiotic resistance, not shaken or stirred

Ryan L Frisch et al. Science. .
No abstract available

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Figures

Figure
Figure. Microenvironment for experimental evolution of antibiotic resistance
(A) The microfluidic chamber of Zhang et al. (1) creates a heterogeneous environment with gradients of antibiotic and nutrients, through which motile bacterial cells migrate. Nutrient medium with the antibiotic cipro flows into the chamber’s left side and medium without antibiotic into the right, creating a cipro gradient from bottom (green, panel B) to top. (B) The steepest gradients occur at “Goldilocks points” where, repeatably, cipro-resistant mutants are initially established/fixed in the evolving populations. (C) The large hexagonal microchamber in A comprises 10μm-deep hexagons connected by 200μm-long channels though which the bacteria migrate towards nutrients and away from cipro, a stressor, until spontaneous resistance arises (red cells in panel D). (D) Close-up of a Goldilocks point. Resistant mutant cells move towards nutrients, irrespective of cipro, and can fix in the population at those points, whereas sensitive cells might not, despite their need for the nutrients.
See this image and copyright information in PMC

Comment on

References

    1. Zhang Q, et al. Science. 2011
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