Characterization of a mouse monoclonal antibody to vimentin by indirect immunofluorescence microscopy and immunoblotting
- PMID: 2195017
Characterization of a mouse monoclonal antibody to vimentin by indirect immunofluorescence microscopy and immunoblotting
Abstract
A murine IgM monoclonal antibody, termed MC15A13G was produced after immunization of Balb/c mouse with Swiss mouse dental papillae. This antibody, characterized by immunoblotting analysis and indirect immunofluorescence microscopy, recognized a 57 Kda protein identified as vimentin in different cell types with no cross reaction with other cytoskeletal proteins.