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. 2011 Jun;22(6):1014-21.
doi: 10.1007/s13361-011-0122-z. Epub 2011 Apr 9.

MALDI mass spectrometric imaging of lipids in rat brain injury models

Joseph A Hankin  1 , Santiago E Farias, Robert M Barkley, Kim Heidenreich, Lauren C Frey, Kei Hamazaki, Hee-Yong Kim, Robert C Murphy
Affiliations

MALDI mass spectrometric imaging of lipids in rat brain injury models

Joseph A Hankin et al. J Am Soc Mass Spectrom. 2011 Jun.

Abstract

Matrix-assisted laser desorption ionization/ionization imaging mass spectrometry (MALDI IMS) with a time-of-flight analyzer was used to characterize the distribution of lipid molecular species in the brain of rats in two injury models. Ischemia/reperfusion injury of the rat brain after bilateral occlusion of the carotid artery altered appearance of the phospholipids present in the hippocampal region, specifically the CA1 region. These brain regions also had a large increase in the ion abundance at m/z 548.5 and collisional activation supported identification of this ion as arising from ceramide (d18:1/18:0), a lipid known to be associated with cellular apoptosis. Traumatic brain injury model in the rat was examined by MALDI IMS and the area of damage also showed an increase in ceramide (d18:1/18:0) and a remarkable loss of signal for the potassium adduct of the most abundant phosphocholine molecular species 16:0/18:1 (PC) with a corresponding increase in the sodium adduct ion. This change in PC alkali attachment ion was suggested to be a result of edema and influx of extracellular fluid likely through a loss of Na/K-ATPase caused by the injury. These studies reveal the value of MALDI IMS to examine tissues for changes in lipid biochemistry and will provide data needed to eventually understand the biochemical mechanisms relevant to tissue injury.

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Figures

Figure 1
Figure 1
MALDI IMS representing palmitoyl-oleoyl phosphatidylcholine [M+H]+ m/z 760.6 in rat brain from (A) a single hemisphere from control animal; (B) a single hemisphere from animal subjected to bilateral ischemia.
Figure 2
Figure 2
MALDI-TOF mass spectra averaged over the hippocampus region of the MALDI image of rat brain from (A) a single hemisphere of control animal; (B) a single hemisphere of animal subjected to bilateral ischemia. Inset:8× expansion of signal intensity scale for mass range m/z 700–1000.
Figure 3
Figure 3
MALDI IMS representing m/z 548.5 ([M+H−H2O]+, Cer (d18:0/18:1)) in rat brain from (A) single hemisphere of control animal; (B) single hemisphere of animal subjected to bilateral ischemia.
Figure 4
Figure 4
Rat brain sections from traumatic brain injury (TBI) model. (A) Tissue section stained with 2,3,5 triphenyl tetrazolium chloride (TTC); B. MALDI IMS representing 16:0/18:1 PC [M+H]+ m/z 760.6; C. MALDI IMS representing 16:0/18:1 PC [M+Na]+ m/z 782.6; D. MALDI IMS representing 16:0/18:1 PC [M+K]+ m/z 798.6.
Figure 5
Figure 5
(A) MALDI IMS representing Cer (d18:0/18:1) [M+H−H2O]+ m/z 548.6 in rat brain with unilateral TBI; (B) CID mass spectrum from m/z 548.5 (on tissue).
See this image and copyright information in PMC

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