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. 2003 Nov:Chapter 1:Unit1.28.
doi: 10.1002/0471141755.ph0128s22.

Characterization of melanocortin receptors

Aaron S Goetz  1 , Diane M Ignar
Affiliations

Characterization of melanocortin receptors

Aaron S Goetz et al. Curr Protoc Pharmacol. 2003 Nov.

Abstract

This unit describes a Scintillation Proximity Assay (SPA) for the measurement of ligand binding to melanocortin receptors (MCRs) using membranes prepared from cell lines stably expressing recombinant MCRs. It provides a facile method for determining the affinity of compounds at MC1R, MC3R, MC4R, or MC5R.

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