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Comparative Study
. 2011 Oct;17(10):MT91-6.
doi: 10.12659/msm.881967.

The clinical use of a fast screening test based on technology of capillary zone electrophoresis (CZE) for identification of Escherichia coli infection in biological material

Jacek Szeliga  1 , Ewa Klodzinska, Marek Jackowski, Bogusław Buszewski
Affiliations
Comparative Study

The clinical use of a fast screening test based on technology of capillary zone electrophoresis (CZE) for identification of Escherichia coli infection in biological material

Jacek Szeliga et al. Med Sci Monit. 2011 Oct.

Abstract

Background: Escherichia coli is a Gram-negative bacterium which is a basic, symbiotic element of the physiological flora of the large intestine of humans and warm-blooded animals. However, in specific cases it may become a very dangerous pathogen (eg, diarrhoea, infection of the urinary tract, lungs, and generalized infections). Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis.

Material/methods: We present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour. Altogether, 30 E. coli-infected wounds and ulcerations were examined, comparing the results obtained by classical culture method with the result of capillary zone electrophoresis (CZE) electropherogram.

Results: The method, which does not require any preparation of the sample, achieved 86.7% sensitivity and 85%specificity in the examined clinical material (infections of surgical wounds).

Conclusions: The obtained results enable reliable, very fast testing for E. coli as a pathogen.

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Figures

Figure 1
Figure 1
Example of an infected wound (appendectomy).
Figure 2
Figure 2
Electropherogram of additional strains in the biological sample, in which conventional method primarily detected only presence of E. coli. The next inoculation showed co-infection of Staphylococcus sp.
Figure 3
Figure 3
Typical electropherograms of biological sample (A, B) and standard (C) with presence of Escherichia coli (arrow).
Figure 4
Figure 4
Selected electropherogram of the biological sample infected with E. coli (+++) and Enterobacter cloacae (+). Clear, pathognomonic signal of E. coli is seen of tm=2.6 minutes.
Figure 5
Figure 5
Spectrum of background (morphotic elements of tissue excretion) – matrix effect.
See this image and copyright information in PMC

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