Evaluation of Antiinflammatory Activity of Centratherum anthelminticum (L) Kuntze Seed

Abstract

In the present study petroleum ether and alcoholic extracts of Centratherum anthelminticum (L) Kuntze seed (100 mg and 200 mg/kg p.o.) were evaluated for antiinflammatory activity in acute and subacute models of inflammation. It was found that both petroleum ether and alcoholic extracts showed significant reduction in paw oedema in carrageenan-induced model. In subchronic inflammatory phase both extracts provoked a significant reduction of transudation phase and too little extent proliferative phase when tested in cotton pellet-induced granuloma model. Both the extracts also reduced alkaline phosphatase activity in serum. The histopathology of granuloma tissue showed significant inhibition of lymphocytes, neutrophils, exudates, necrosis and giant cell when compared with control without ulcerogenic effect. The results suggest that petroleum ether and alcoholic extracts may exert antiinflammatory activity through prostaglandin inhibition, reduced myeloperoxidase and antitransudation.

Keywords: Antiinflammatory; Centratherum anthelminticum; alkaline phosphatase; myeloperoxidase.

Fig. 1

Histological sections of granuloma tissue (a and b). control group; (c) standard group; (d) petroleum ether extract 100 mg/kg group; (e) petroleum ether extract 200 mg/kg group; (f) alcohol extract 100 mg/kg group; (g) alcohol extract 200 mg/kg group. (a) Histological section of granuloma tissue in control group showing severe acute inflammation with necrosis, exudates and fats. (b) Histological section of granuloma tissue in control group showing acute inflammation with giant cells and lymphocyte. (c) Histological section of granuloma tissue in standard group showing mild chronic inflammation with granulation tissue and blood vessels. (d) Histological section of granuloma tissue in petroleum ether extract 100 mg/kg group showing mild-moderate chronic inflammation with exudates and necrosis. (e) histological section of granuloma tissue in petroleum ether extract 200 mg/kg group showing mild chronic inflammation with granulation and blood vessels. (f) Histological section of granuloma tissue in alcohol extract 100 mg/kg group showing moderate to severe acute inflammation with exudates, necrosis (g) Histological section of granuloma tissue in alcohol extract 200 mg/kg group moderate chronic inflammation with exudates.