[Total protein analysis by two-dimensional electrophoresis in cysticerci of Taenia solium and Taenia asiatica]
[Article in
Chinese]
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- PMID: 21970107
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[Total protein analysis by two-dimensional electrophoresis in cysticerci of Taenia solium and Taenia asiatica]
[Article in
Chinese]
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi.
2011 Jun.
Abstract
Two 20-day-old three-way crossed hybrid pigs were infected with 80000 Taenia solium or T. asiatica eggs, respectively. Immature cysticerci of the two species in liver were collected at 40 days after infection. The total proteins were separated by two-dimensional electrophoresis, and differentially expressed proteins were analyzed by Image-Master 2D Platinum 6.0 software. The results showed that there were (236 +/- 12) and (231 +/- 14) protein spots in 2D electrophoresis gel images of T. solium and T. asiatica, respectively, with 3 proteins up-regulated and 7 proteins down-regulated in T. solium cysticercus by 2-fold or more compared with those in T. asiatica cysticercus.
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