The influence of different adjuvants on the production of IgD and IgE antibodies

Abstract

The aim of this study was to investigate the relationship between the synthesis of IgD and IgE antibodies upon the administration of antigen. Different adjuvants were used in order to find the optimal way of inducing in rats the synthesis of IgD and IgE antibodies. Ovalbumin was used as antigen. Fair amounts of IgE antibodies were produced using Al(OH)3 or Bordetella pertussis and Al(OH)3 as adjuvant. Only small quantities of IgE antibodies were formed when either Freund's complete adjuvant (FCA) or no adjuvant were used. IgD antibodies are formed upon the immunization with ovalbumin and FCA or B. pertussis in combination with Al(OH)3. Using Al(OH)3 alone as adjuvant or antigen alone resulted in only very minute quantities of specific IgD. Total serum IgE levels raised in the rats immunized with Al(OH)3 or B. pertussis and Al(OH)3. Total serum IgD was increased after immunization with ovalbumin and B. pertussis in combination with Al(OH)3.