Galactomannan antigenemia after infusion of gluconate-containing Plasma-Lyte

Abstract

We demonstrated that sodium gluconate was the factor causing false-positive galactomannan (GM) antigenemia of Plasma-Lyte hydration solution. Infusion of sodium gluconate-containing solution but not gluconate-free Plasma-Lyte resulted in positive serum GM antigenemia. Serum GM concentrations also correlated with the volume and in vitro concentrations of GM within gluconate-containing solutions of infused Plasma-Lyte.

Fig. 1

Expression of GM antigen following the intravenous infusion of different formulations and lots of Plasma-Lyte solutions. (A) Combined concentration-time curve of single 30-ml/kg infusions each of gluconate-containing Plasma-Lyte A (lot C738476; GMI, 7.26 ± 0.01) and gluconate-containing Plasma-Lyte 148 (lot C730549; GMI, 8.17 ± 0.15) given over 1 h. (B) Single-dose 30-ml/kg infusion of a solution of Plasma-Lyte 56 (GMI, 0.16 ± 0.01) without gluconate administered over 1 h. (C) Combined concentration-time curve after the administration of multiple-dose gluconate-containing Plasma-Lyte A (lot C738476) and Plasma-Lyte 148 (lot C730549) infusions given over 1 h performed on day 8. (D) Multiple infusions of a solution of Plasma-Lyte 56 without gluconate given over 1 h performed on day 8. Values are presented as means ± stand errors of the means. *, P < 0.0001 (Kruskal-Wallis test).

Fig. 2

Expression of GM antigen following intravenous infusion of gluconate-containing Plasma-Lyte A (lot C689893; GMI 4.18 ± 0.01). (A) A 10-ml/kg infusion administered over 10 min. (B) A 30-ml/kg infusion administered over 1 h. Values are presented as means ± stand errors of the means. ¶, P = 0.0002 (Mann-Whitney test); *, P < 0.0001 (Kruskal-Wallis test).