Discovery and genomic characterization of a novel ovine partetravirus and a new genotype of bovine partetravirus

Abstract

Partetravirus is a recently described group of animal parvoviruses which include the human partetravirus, bovine partetravirus and porcine partetravirus (previously known as human parvovirus 4, bovine hokovirus and porcine hokovirus respectively). In this report, we describe the discovery and genomic characterization of partetraviruses in bovine and ovine samples from China. These partetraviruses were detected by PCR in 1.8% of bovine liver samples, 66.7% of ovine liver samples and 71.4% of ovine spleen samples. One of the bovine partetraviruses detected in the present samples is phylogenetically distinct from previously reported bovine partetraviruses and likely represents a novel genotype. The ovine partetravirus is a novel partetravirus and phylogenetically most related to the bovine partetraviruses. The genome organization is conserved amongst these viruses, including the presence of a putative transmembrane protein encoded by an overlapping reading frame in ORF2. Results from the present study provide further support to the classification of partetraviruses as a separate genus in Parvovirinae.

Figure 1. Phylogenetic tree constructed from the full-length aa sequences of NS1 of partetraviruses and other parvoviruses.

Approximate likelihood SH-like support values >0.50 are shown besides major branches. NS1 aa sequences of bovine partetravirus HK1 to HK3 are identical to that of bovine partetravirus HK4 and not shown. Scale bar indicates 0.5 inferred substitutions per site. Abbreviations: PPtV, porcine partetravirus; BPtV, bovine partetravirus; OPtV, ovine partetravirus; PV, parvovirus; AAV, adeno-associated virus; BoV, bocavirus.

Figure 2. Phylogenetic tree constructed from the full-length aa sequences of VP1 of partetraviruses and other parvoviruses.

Approximate likelihood SH-like support values >0.50 are shown besides major branches. VP1 aa sequences of bovine partetravirus HK1 to HK3 are identical to that of bovine partetravirus HK4 and not shown. Scale bar indicates 0.2 inferred substitutions per site. Abbreviations: PPtV, porcine partetravirus; BPtV, bovine partetravirus; OPtV, ovine partetravirus; PV, parvovirus; AAV, adeno-associated virus; BoV, bocavirus.