Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Oct 7:11:86.
doi: 10.1186/1472-6882-11-86.

Tannin extracts from immature fruits of Terminalia chebula Fructus Retz. promote cutaneous wound healing in rats

Affiliations

Tannin extracts from immature fruits of Terminalia chebula Fructus Retz. promote cutaneous wound healing in rats

Kun Li et al. BMC Complement Altern Med. .

Abstract

Background: Tannins extracted from immature fruits of Terminalia chebula Fructus Retz. are considered as effective components promoting the process of wound healing. The objective of this study is to explore the optimal extraction and purification technology (OEPT) of tannins, while studying the use of this drug in the treatment of a cutaneous wound of rat as well as its antibacterial effects.

Methods: The content of tannin extracts was measured by the casein method, and antibacterial ability was studied by the micro-dilution method in vitro. In wound healing experiment, animals in group Ⅰ, Ⅱ and Ⅲ were treated with vaseline ointment, tannin extracts (tannin content: 81%) and erythromycin ointment, respectively (5 mg of ointment were applied on each wound). To evaluate the process of wound healing, selected pharmacological and biochemical parameters were applied.

Results: After optimal extraction and purification, content of tannin extracts was increased to 81%. Tannin extracts showed the inhibition of Staphylococcus aureus and Klebsiella Pneumonia in vitro. After excision of wounds, on days 7 and 10, the percent of wound contraction of group Ⅱ was higher than that of group Ⅰ. After being hurt with wounds, on days 3, 7, and 10, the wound healing quality of group Ⅱ was found to be better than that of group Ⅰ in terms of granulation formation and collagen organization. After wound creation, on day 3, the vascular endothelial growth factor expression of group Ⅱ was higher than that of group Ⅰ.

Conclusion: The results suggest that tannin extracts from dried immature fruits of Terminalia chebula Fructus Retz. can promote cutaneous wound healing in rats, probably resulting from a powerful anti-bacterial and angiogenic activity of the extracts.

PubMed Disclaimer

Figures

Figure 1
Figure 1
The calibration curve of gallic acid. linear range (1~10 μg/ml), Regression equation(Y = aX + b): y = 52.496x + 0.0163, R2 = 0.9993(n = 6)
Figure 2
Figure 2
Morphology of Staphylococcus aureus and Klebsiella Pneumonia under scanning electron microscope. A the normal morphology of Staphylococcus aureus; B the morphology of Staphylococcus aureus on 24 h; C the normal morphology of Klebsiella Pneumonia; D the morphology of Klebsiella Pneumonia on 24 h
Figure 3
Figure 3
Histological compare in the character of wound healing on day 3. (100 × magnification). A: groupⅠ; B: groupⅡ; C: groupⅢ
Figure 4
Figure 4
Immunohistochemical examination of VEGFA expression on day 3. (400 × magnification). A: groupⅠ; B: groupⅡ; C: group Ⅲ
Figure 5
Figure 5
VEGFA mRNA expression on day 3. (*P < 0.05 as compared with groupⅠ)

References

    1. Sen CK, Khanna S, Gordillo G, Bagchi D, Bagchi M, Roy S. Oxygen, oxidants, and antioxidants in wound healing: an emerging paradigm. Ann N Y Acad Sci. 2002;957:239–249. doi: 10.1111/j.1749-6632.2002.tb02920.x. - DOI - PubMed
    1. Fernandez O, Capdevila JZ, Dalla G, Melchor G. Efficacy of Rhizophora mangle aqueous bark extract in the healing of open surgical wounds. Fitoterapia. 2002;73:564–568. doi: 10.1016/S0367-326X(02)00229-0. - DOI - PubMed
    1. Saleem A, Ahotupa M, Pihlaja K. Total phenolics concentration and antioxidant potential of extracts of medicinal plants of Pakistan. Z Naturforsch C. 2001;56(11-12):973–978. - PubMed
    1. Kusirisin W, Srichairatanakool S, Lerttrakarnnon P, Lailerd N, Suttajit M, Jaikang C, Chaiyasut C. Antioxidative activity, polyphenolic content and anti-glycation effect of some Thai medicinal plants traditionally used in diabetic patients. Med Chem. 2009;5(2):139–147. doi: 10.2174/157340609787582918. - DOI - PubMed
    1. Nariya M, Shukla V, Jain S, Ravishankar B. Comparison of enteroprotective efficacy of triphala formulations (Indian Herbal Drug) on methotrexate-induced small intestinal damage in rats. Phytother Res. 2009;23(8):1092–1098. doi: 10.1002/ptr.2744. - DOI - PubMed

Publication types

MeSH terms