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. 2011 Dec;87(3):375-7.
doi: 10.1016/j.mimet.2011.08.019. Epub 2011 Sep 25.

Measurement of bacterial volume by transmission-through-dye imaging

Suzanne L Lababidi  1 , Mariana Pelts, Moumita Moitra, Laura G Leff, Michael A Model
Affiliations

Measurement of bacterial volume by transmission-through-dye imaging

Suzanne L Lababidi et al. J Microbiol Methods. 2011 Dec.

Abstract

Transmission-through-dye (TTD) microscopy makes possible direct measurement of bacterial volume, irrespective of cell shape. The technique can be realized on any brightfield microscope and is applicable to bacteria of all shapes. TTD imaging requires that intact bacteria be immobilized on a flat transparent surface, such as a glass coverslip.

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