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. 2011 Oct 9;8(12):1047-9.
doi: 10.1038/nmeth.1744.

Live-cell 3D super-resolution imaging in thick biological samples

Francesca Cella Zanacchi  1 , Zeno Lavagnino, Michela Perrone Donnorso, Alessio Del Bue, Laura Furia, Mario Faretta, Alberto Diaspro
Affiliations

Live-cell 3D super-resolution imaging in thick biological samples

Francesca Cella Zanacchi et al. Nat Methods. .

Abstract

We demonstrate three-dimensional (3D) super-resolution live-cell imaging through thick specimens (50-150 μm), by coupling far-field individual molecule localization with selective plane illumination microscopy (SPIM). The improved signal-to-noise ratio of selective plane illumination allows nanometric localization of single molecules in thick scattering specimens without activating or exciting molecules outside the focal plane. We report 3D super-resolution imaging of cellular spheroids.

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