doi: 10.1016/0005-2787(79)90134-5.
The purification of nuclease-free T4-RNA ligase
- PMID: 219895
- DOI: 10.1016/0005-2787(79)90134-5
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The purification of nuclease-free T4-RNA ligase
Biochim Biophys Acta.
.
Abstract
RNA ligase has been highly purified in good yields from bacteriophage T4-infected Escherichia coli by a rapid and reproducible procedure. The enzyme is free of phosphomonoesterase and ribonuclease activities and is therefore suitable for the synthesis of oligoribonucleotides and for the labeling of the 3'-terminus of RNA. Greater than 90% of the protein in the enzyme preparation migrates as a single band on gradient polyacrylamide gels containing sodium dodecyl sulfate during electrophoresis. For use as a DNA synthesis reagent the enzyme may be reliably freed of deoxyribonuclease activity by an additional chromatographic procedure using a commercially avialable resin.
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