Intraspecific variation in the thermal plasticity of mitochondria in killifish

Abstract

Populations of the Atlantic killifish (Fundulus heteroclitus) inhabit salt marshes and estuaries along the eastern coast of North America from Newfoundland to northern Florida, and are thus exposed to a large range of temperatures. Previous studies have shown higher whole-organism metabolic rates in the northern subspecies (F. h. macrolepidotus) compared with the southern subspecies (F. h. heteroclitus) of these fish. Here, we examine phenotypic plasticity in the response to cold temperatures between the two subspecies by acclimating fish to 5, 15 and 25°C and comparing several mitochondrial and muscle properties. The relative area of oxidative muscle versus glycolytic muscle fibers was greater in the northern subspecies at the 5 and 15°C acclimation temperatures. However, there were no differences in capillary density between the two subspecies or at different temperatures. Mitochondrial volume and surface densities increased in response to cold temperature acclimation in red and white muscle, but only in the northern killifish. Citrate synthase activities also increased in the northern killifish at 5 and 15°C. The ratio of calculated [free ADP] to [ATP] increased in the 5°C acclimated southern killifish but not in the northern killifish at 5°C when compared with the 15°C acclimation group, suggesting that there are differences in adenylate signaling for mitochondrial respiration between subspecies at low temperature. Taken together, our data indicate that the northern subspecies have a greater ability to increase mitochondrial capacity at colder temperatures compared with the southern subspecies, providing one of the few examples of intraspecific variation in phenotypic plasticity in mitochondrial amount in response to cold temperatures.