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. 2012 Jan;91(1):22-27.
doi: 10.1111/j.1600-0412.2011.01299.x. Epub 2011 Nov 9.

In vitro maturation: a five-year experience

Sabine Roesner  1 , Michael VON Wolff  2 , Ingeborg Eberhardt  1 , Petra Beuter-Winkler  1 , Bettina Toth  1 , Thomas Strowitzki  1
Affiliations
Free article

In vitro maturation: a five-year experience

Sabine Roesner et al. Acta Obstet Gynecol Scand. 2012 Jan.
Free article

Abstract

Objective: Although some patients may benefit from reduced follicle-stimulating hormone (FSH) application, in-vitro maturation (IVM) belongs to the rare treatment options in assisted reproduction. We summarize our five-year IVM experience.

Design: Retrospective, observational study.

Setting: Reproductive Medicine, University Hospital.

Sample: 115 patients with polycystic ovary syndrome (PCOS) as well as patients after ovarian hyperstimulation syndrome (OHSS) from February 2005 to December 2009.

Material and methods: Stimulation started between day 3-10 of the menstrual cycle and FSH dosage was 125IU/day over three days. Ovulation was induced on the third day of FSH injection or one day afterwards and oocyte retrieval was performed 33-38 hours later. Oocytes were cultivated for 24 hours in IVM medium. Fertilization was carried out one day after oocyte retrieval and embryo transfer two days afterwards.

Main outcome measures: Pregnancy rates.

Results: 115 patients were included and 215 oocyte retrievals (intracytoplasmic sperm injection: n=125, 59%; in vitro fertilization: n=73, 34.4%) with 177 embryo transfers performed. The main reasons for IVM were: PCOS (71.7%) and OHSS (15.0%). Mean number of oocytes was 8.9/oocyte retrieval with 5.9 (64%) becoming mature, 2.8 (45.1%) being fertilized and 2.1 transferred. Pregnancy rate per transfer was 15.3% (n=27) with 13 live births (7.3%), one intrauterine death (0.6%), four miscarriages (2.3%) and nine biochemical pregnancies (5.1%). In 61 cases, fertilized oocytes were frozen and 32 cryotransfers were performed, resulting in three pregnancies.

Conclusions: Although the pregnancy rate was low, IVM is very convenient for patients due to low FSH dosages and few appointments at low cost.

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