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. 2011 Dec;49(12):4341-2.
doi: 10.1128/JCM.05454-11. Epub 2011 Oct 12.

Xpert MTB/RIF assay for diagnosis of pleural tuberculosis

Sven O Friedrich  1 , Florian von Groote-Bidlingmaier, Andreas H Diacon
Affiliations

Xpert MTB/RIF assay for diagnosis of pleural tuberculosis

Sven O Friedrich et al. J Clin Microbiol. 2011 Dec.

Abstract

We prospectively investigated the diagnostic utility of the Xpert MTB/RIF (Mycobacterium tuberculosis/rifampin [RIF] resistance) assay in 20 cases with confirmed tuberculous pleural effusion. The sensitivity and specificity of the Xpert assay in pleural fluid were 25% and 100%, respectively. All cases positive by the Xpert assay were also positive by pleural fluid culture.

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Figures

Fig. 1.
Fig. 1.
Effect of frozen sample storage on CT value of probe B. The storage of sputum pellet samples at −80°C for 30 days in PBS (pH 6.8) without glycerol increased the calculated mean CT value of probe B in samples determined to be M. tuberculosis positive by the Xpert assay (ΔCT = 2.11, n = 11). This difference is statistically significant (*, P = 0.0021, paired two-tailed Student's t test).
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References

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    1. Friedrich S. O., et al. 2011. Suitability of Xpert MTB/RIF and genotype MTBDRplus for patient selection for a tuberculosis clinical trial. J. Clin. Microbiol. 49: 2827–2831 - PMC - PubMed

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