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Comparative Study
. 2011 Dec;49(12):4314-8.
doi: 10.1128/JCM.05788-11. Epub 2011 Oct 12.

Species identification of clinical isolates of anaerobic bacteria: a comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry systems

Ulrik Stenz Justesen  1 , Anette Holm, Elisa Knudsen, Line Bisgaard Andersen, Thøger Gorm Jensen, Michael Kemp, Marianne Nielsine Skov, Bente Gahrn-Hansen, Jens Kjølseth Møller
Affiliations
Comparative Study

Species identification of clinical isolates of anaerobic bacteria: a comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry systems

Ulrik Stenz Justesen et al. J Clin Microbiol. 2011 Dec.

Erratum in

  • J Clin Microbiol. 2012 Feb;50(2):542

Abstract

We compared two matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) systems (Shimadzu/SARAMIS and Bruker) on a collection of consecutive clinically important anaerobic bacteria (n = 290). The Bruker system had more correct identifications to the species level (67.2% versus 49.0%), but also more incorrect identifications (7.9% versus 1.4%). The system databases need to be optimized to increase identification levels. However, MALDI-TOF MS in its present version seems to be a fast and inexpensive method for identification of most clinically important anaerobic bacteria.

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