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. 2011 Oct 16;8(12):1044-6.
doi: 10.1038/nmeth.1734.

Super-resolution 3D microscopy of live whole cells using structured illumination

Lin Shao  1 , Peter Kner, E Hesper Rego, Mats G L Gustafsson
Affiliations

Super-resolution 3D microscopy of live whole cells using structured illumination

Lin Shao et al. Nat Methods. .

Abstract

Three-dimensional (3D) structured-illumination microscopy (SIM) can double the lateral and axial resolution of a wide-field fluorescence microscope but has been too slow for live imaging. Here we apply 3D SIM to living samples and record whole cells at up to 5 s per volume for >50 time points with 120-nm lateral and 360-nm axial resolution. We demonstrate the technique by imaging microtubules in S2 cells and mitochondria in HeLa cells.

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References

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