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. 2012 Jan;53(1):168-74.
doi: 10.1194/jlr.P018457. Epub 2011 Oct 14.

Serum paraoxonase-3 concentration in HIV-infected patients. Evidence for a protective role against oxidation

Gerard Aragonès  1 , Anabel García-Heredia, Marta Guardiola, Anna Rull, Raúl Beltrán-Debón, Judit Marsillach, Carlos Alonso-Villaverde, Bharti Mackness, Michael Mackness, Juan Pedro-Botet, Pedro Pardo-Reche, Jorge Joven, Jordi Camps
Affiliations

Serum paraoxonase-3 concentration in HIV-infected patients. Evidence for a protective role against oxidation

Gerard Aragonès et al. J Lipid Res. 2012 Jan.

Abstract

We investigated the influence of the HIV infection on serum paraoxonase-3 (PON3) concentration and assessed the relationships with lipoprotein-associated abnormalities, immunological response, and accelerated atherosclerosis. We studied 207 HIV-infected patients and 385 healthy volunteers. Serum PON3 was determined by in-house ELISA, and PON3 distribution in lipoproteins was investigated by fast-performance liquid chromatography (FPLC). Polymorphisms of the PON3 promoter were analyzed by the Iplex Gold MassArray(TM) method. PON3 concentrations were increased (about three times) in HIV-infected patients with respect to controls (P < 0.001) and were inversely correlated with oxidized LDL levels (P = 0.038). Long-term use of nonnucleoside reverse transcriptase inhibitor (NNRTI)-based antiretroviral therapy was associated with a decrease of PON3 concentrations. In a multivariate linear regression analysis, these relationships were still strong when the main confounding covariates were considered. PON3 was mainly found in HDL in HIV-infected patients, but a substantial amount of the protein was detected in LDL particles. This study reports for the first time an important increase in serum PON3 concentrations in HIV-infected patients that is associated with their oxidative status and their treatment with NNRTI. Long-term, prospective studies are needed to confirm the possible influence of this enzyme on the course of this disease and its possible utility as an analytical biomarker.

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Figures

Fig. 1.
Fig. 1.
(A) Serum PON3 concentrations in control subjects and HIV-infected patients. (B) ROC plot for serum PON3 concentration measurement in HIV-infected and noninfected subjects. AUC, Area-under-the curve; CI, Confidence interval. (C) Relationship between serum PON3 concentrations and oxidized LDL levels (ox-LDL) in HIV-infected patients.
Fig. 2.
Fig. 2.
FPLC lipoprotein fractionation and PON3 lipoprotein distribution in the control and the HIV-infected pools.
Fig. 3.
Fig. 3.
Relationships among serum PON3 concentrations, β-2-microglobulin concentrations, and the duration of nonnucleoside reverse transcriptase inhibitors (NNRTI) in HIV-infected patients.
See this image and copyright information in PMC

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