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. 2012 Jan;29(1):11-4.
doi: 10.1007/s10815-011-9647-0. Epub 2011 Oct 19.

Value of sperm chromatin dispersion test in couples with unexplained recurrent abortion

Forouzan Absalan  1 , Alireza Ghannadi, Marjaneh Kazerooni, Rostami Parifar, Fatemeh Jamalzadeh, Sahar Amiri
Affiliations

Value of sperm chromatin dispersion test in couples with unexplained recurrent abortion

Forouzan Absalan et al. J Assist Reprod Genet. 2012 Jan.

Abstract

Objective: To evaluate and compare standard sperm parameters and sperm chromatin integrity by sperm chromatin dispersion test (SCD) in ejaculates from men whose partners have a history of recurrent pregnancy loss and from control group of fertile men.

Methods: Thirty couples with unexplained recurrent abortion (case group) and 30 fertile couples (control group) referring to Shiraz infertility center were included. Sperm parameters were assessed in semen samples from two groups and then staining with SCD procedure. The results were analyzed by performing ANOVA and Tukey(,)s tests.

Results: In control group, nucleoids with big (65.93 ± 2.35), small (12.4 ± 0.60) and without halo (11.6 ± 0.50) showed significant difference with case group (41.40 ± 1.43), (21.16 ± 1.11) and (23.26 ± 1.10) respectively. In the RPL group spermatozoa with high percentage of abnormal parameters (morphology and motility) was observed (p ≤ 0.05).

Conclusion: This study strengthens the current literature associating sperm quality with recurrent pregnancy loss, and emphasizes the important of evaluating male factor by tests such as SCD in addition to conventional sperm parameters.

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Figures

Fig. 1
Fig. 1
Nucleoides from human sperm cells obtained with the SCD procedure: Nucleoides with big halo of DNA dispersion (a), medium sized (b), small (c) and without halo and degenerated
See this image and copyright information in PMC

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