Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Dec;49(12):4347-8.
doi: 10.1128/JCM.01272-11. Epub 2011 Oct 19.

Significant finding of Bordetella holmesii DNA in nasopharyngeal samples from French patients with suspected pertussis

Elisabeth Njamkepo  1 , Stéphane Bonacorsi, Monique Debruyne, Sophie Anne Gibaud, Sophie Guillot, Nicole Guiso
Affiliations

Significant finding of Bordetella holmesii DNA in nasopharyngeal samples from French patients with suspected pertussis

Elisabeth Njamkepo et al. J Clin Microbiol. 2011 Dec.

Abstract

Pertussis is routinely diagnosed with real-time PCR based on insertion sequence IS481, which is not specific for Bordetella pertussis. We conducted a retrospective study using real-time PCRs specific for Bordetella pertussis and for Bordetella holmesii on 177 samples positive for IS481 PCR. Bordetella holmesii DNA was detected in 20.3% samples collected from adolescents and adults.

PubMed Disclaimer

Comment in

References

    1. Andre P., et al. 2008. Comparison of serological and real-time PCR assays to diagnose Bordetella pertussis infection in 2007. J. Clin. Microbiol. 46: 1672–1677 - PMC - PubMed
    1. Antila M., et al. 2006. Bordetella holmesii DNA is not detected in nasopharyngeal swabs from Finnish and Dutch patients with suspected pertussis. J. Med. Microbiol. 55: 1043–1051 - PubMed
    1. Bouchez V., et al. 2009. First report and detailed characterization of B. pertussis isolates not expressing pertussis toxin or pertactin. Vaccine 27: 6034–6041 - PubMed
    1. Guthrie J. L., Robertson A. V., Tang P., Jamieson F., Drews S. J. 2010. Novel duplex real-time PCR assay detects Bordetella holmesii in specimens from patients with pertussis-like symptoms in Ontario, Canada. J. Clin. Microbiol. 48: 1435–1437 - PMC - PubMed
    1. Guthrie J. L., et al. 2008. Use of Bordetella pertussis BP3385 to establish a cutoff value for an IS481-targeted real-time PCR assay. J. Clin. Microbiol. 46: 3798–3799 - PMC - PubMed

Publication types

MeSH terms

LinkOut - more resources